Regulation of synaptic vesicle recycling by complex formation between intersectin 1 and the clathrin adaptor complex AP2

A. Pechstein, J. Bacetic, A. Vahedi-Faridi, K. Gromova, A. Sundborger, N. Tomlin, G. Krainer, O. Vorontsova, J. G. Schafer, S. G. Owe, M. A. Cousin, W. Saenger, O. Shupliakov, V. Haucke

Research output: Contribution to journalArticlepeer-review


Clathrin-mediated synaptic vesicle (SV) recycling involves the spatiotemporally controlled assembly of clathrin coat components at phosphatidylinositiol (4, 5)-bisphosphate [ PI(4,5)P-2]-enriched membrane sites within the periactive zone. Such spatiotemporal control is needed to coordinate SV cargo sorting with clathrin/AP2 recruitment and to restrain membrane fission and synaptojanin-mediated uncoating until membrane deformation and clathrin coat assembly are completed. The molecular events underlying these control mechanisms are unknown. Here we show that the endocytic SH3 domain-containing accessory protein intersectin 1 scaffolds the endocytic process by directly associating with the clathrin adaptor AP2. Acute perturbation of the intersectin 1-AP2 interaction in lamprey synapses in situ inhibits the onset of SV recycling. Structurally, complex formation can be attributed to the direct association of hydrophobic peptides within the intersectin 1 SH3A-B linker region with the "side sites" of the AP2 alpha- and beta-appendage domains. AP2 appendage association of the SH3A-B linker region inhibits binding of the inositol phosphatase synaptojanin 1 to intersectin 1. These data identify the intersectin-AP2 complex as an important regulator of clathrin-mediated SV recycling in synapses.
Original languageEnglish
Pages (from-to)4206-4211
Number of pages6
JournalProceedings of the National Academy of Sciences (PNAS)
Issue number9
Publication statusPublished - Mar 2010


  • endocytosis
  • synapse
  • scaffolding proteins
  • appendage
  • synaptojanin


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