Regulation of the hDlg/hScrib/Hugl-1 tumour suppressor complex

Paola Massimi, Nisha Narayan, Miranda Thomas, Noor Gammoh, Susanne Strand, Dennis Strand, Lawrence Banks

Research output: Contribution to journalArticlepeer-review

Abstract

The proper function of the Scribble tumour suppressor complex is dependent upon the correct localisation of its components. Previously we observed dynamic relocalisation of the hDlg component under conditions of osmotic stress. We now show that the other two components of the complex, hScrib and Hugl-1 display similar patterns of expression. We demonstrate, by shRNA ablation of hScrib expression, that hDlg and Hugl-1 are in part dependent upon hScrib for their correct localization. However under conditions of osmotic stress this apparent dependency no longer exists: hDlg and Hugl-1 localise to cell membranes independently of hScrib. We also demonstrate an interaction between the three components of the hScrib complex and the tSNARE syntaxin 4, and show that correct localization of the Scrib complex is in part tSNARE dependent. This is the first detailed analysis of the co-localisation and function of the hScrib complex in mammalian cells and demonstrates a direct link between the control of the hScrib complex and vesicle transport pathways.
Original languageEnglish
Pages (from-to)3306-17
Number of pages12
JournalExperimental Cell Research
Volume314
Issue number18
DOIs
Publication statusPublished - 1 Nov 2008

Keywords

  • Adaptor Proteins, Signal Transducing
  • Animals
  • Blotting, Western
  • Cell Line
  • Cytoskeletal Proteins
  • Gene Expression Regulation
  • Humans
  • Membrane Proteins
  • Multiprotein Complexes
  • Osmolar Concentration
  • Protein Transport
  • Qa-SNARE Proteins
  • RNA Interference
  • Signal Transduction
  • Sorbitol
  • Transport Vesicles
  • Tumor Suppressor Proteins

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