Regulation of the miR-212/132 locus by MSK1 and CREB in response to neurotrophins

Judit Remenyi; Christopher J Hunter; Christian Cole; Hideaki Ando; Soren Impey; Claire E Monk; Kirsty J Martin; Geoffrey J Barton; Gyorgy Hutvagner; J Simon C Arthur

doi:10.1042/BJ20100024

Regulation of the miR-212/132 locus by MSK1 and CREB in response to neurotrophins

Judit Remenyi, Christopher J Hunter, Christian Cole, Hideaki Ando, Soren Impey, Claire E Monk, Kirsty J Martin, Geoffrey J Barton, Gyorgy Hutvagner, J Simon C Arthur

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Abstract / Description of output

Neurotrophins are growth factors that are important in neuronal development and survival as well as synapse formation and plasticity. Many of the effects of neurotrophins are mediated by changes in protein expression as a result of altered transcription or translation. To determine whether neurotrophins regulate the production of microRNAs (miRNAs), small RNA species that modulate protein translation or mRNA stability, we used deep sequencing to identify BDNF (brain-derived neurotrophic factor)-induced miRNAs in cultured primary cortical mouse neurons. This revealed that the miR-212/132 cluster contained the miRNAs most responsive to BDNF treatment. This cluster was found to produce four miRNAs: miR-132, miR-132*, miR-212 and miR-212*. Using specific inhibitors, mouse models and promoter analysis we have shown that the regulation of the transcription of the miR-212/132 miRNA cluster and the miRNAs derived from it are regulated by the ERK1/2 (extracellular-signal-regulated kinase 1/2) pathway, via both MSK (mitogen and stress-activated kinase)-dependent and -independent mechanisms.

Original language	English
Pages (from-to)	281-91
Number of pages	11
Journal	Biochemical Journal
Volume	428
Issue number	2
DOIs	https://doi.org/10.1042/BJ20100024
Publication status	Published - 1 Jun 2010

Keywords / Materials (for Non-textual outputs)

Animals
Base Sequence
Benzamides
Blotting, Northern
Brain-Derived Neurotrophic Factor
Cells, Cultured
Cyclic AMP Response Element-Binding Protein
Mice
Mice, Inbred C57BL
Mice, Knockout
Mice, Transgenic
MicroRNAs
Mitogen-Activated Protein Kinase 1
Mitogen-Activated Protein Kinase 3
Molecular Sequence Data
Neurons
Nucleic Acid Amplification Techniques
Phosphorylation
Polymerase Chain Reaction
Ribosomal Protein S6 Kinases, 90-kDa
Sequence Homology, Nucleic Acid

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10.1042/BJ20100024

Cite this

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title = "Regulation of the miR-212/132 locus by MSK1 and CREB in response to neurotrophins",

abstract = "Neurotrophins are growth factors that are important in neuronal development and survival as well as synapse formation and plasticity. Many of the effects of neurotrophins are mediated by changes in protein expression as a result of altered transcription or translation. To determine whether neurotrophins regulate the production of microRNAs (miRNAs), small RNA species that modulate protein translation or mRNA stability, we used deep sequencing to identify BDNF (brain-derived neurotrophic factor)-induced miRNAs in cultured primary cortical mouse neurons. This revealed that the miR-212/132 cluster contained the miRNAs most responsive to BDNF treatment. This cluster was found to produce four miRNAs: miR-132, miR-132*, miR-212 and miR-212*. Using specific inhibitors, mouse models and promoter analysis we have shown that the regulation of the transcription of the miR-212/132 miRNA cluster and the miRNAs derived from it are regulated by the ERK1/2 (extracellular-signal-regulated kinase 1/2) pathway, via both MSK (mitogen and stress-activated kinase)-dependent and -independent mechanisms.",

keywords = "Animals, Base Sequence, Benzamides, Blotting, Northern, Brain-Derived Neurotrophic Factor, Cells, Cultured, Cyclic AMP Response Element-Binding Protein, Mice, Mice, Inbred C57BL, Mice, Knockout, Mice, Transgenic, MicroRNAs, Mitogen-Activated Protein Kinase 1, Mitogen-Activated Protein Kinase 3, Molecular Sequence Data, Neurons, Nucleic Acid Amplification Techniques, Phosphorylation, Polymerase Chain Reaction, Ribosomal Protein S6 Kinases, 90-kDa, Sequence Homology, Nucleic Acid",

author = "Judit Remenyi and Hunter, {Christopher J} and Christian Cole and Hideaki Ando and Soren Impey and Monk, {Claire E} and Martin, {Kirsty J} and Barton, {Geoffrey J} and Gyorgy Hutvagner and Arthur, {J Simon C}",

year = "2010",

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doi = "10.1042/BJ20100024",

language = "English",

volume = "428",

pages = "281--91",

journal = "Biochemical Journal",

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T1 - Regulation of the miR-212/132 locus by MSK1 and CREB in response to neurotrophins

AU - Remenyi, Judit

AU - Hunter, Christopher J

AU - Cole, Christian

AU - Ando, Hideaki

AU - Impey, Soren

AU - Monk, Claire E

AU - Martin, Kirsty J

AU - Barton, Geoffrey J

AU - Hutvagner, Gyorgy

AU - Arthur, J Simon C

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N2 - Neurotrophins are growth factors that are important in neuronal development and survival as well as synapse formation and plasticity. Many of the effects of neurotrophins are mediated by changes in protein expression as a result of altered transcription or translation. To determine whether neurotrophins regulate the production of microRNAs (miRNAs), small RNA species that modulate protein translation or mRNA stability, we used deep sequencing to identify BDNF (brain-derived neurotrophic factor)-induced miRNAs in cultured primary cortical mouse neurons. This revealed that the miR-212/132 cluster contained the miRNAs most responsive to BDNF treatment. This cluster was found to produce four miRNAs: miR-132, miR-132*, miR-212 and miR-212*. Using specific inhibitors, mouse models and promoter analysis we have shown that the regulation of the transcription of the miR-212/132 miRNA cluster and the miRNAs derived from it are regulated by the ERK1/2 (extracellular-signal-regulated kinase 1/2) pathway, via both MSK (mitogen and stress-activated kinase)-dependent and -independent mechanisms.

AB - Neurotrophins are growth factors that are important in neuronal development and survival as well as synapse formation and plasticity. Many of the effects of neurotrophins are mediated by changes in protein expression as a result of altered transcription or translation. To determine whether neurotrophins regulate the production of microRNAs (miRNAs), small RNA species that modulate protein translation or mRNA stability, we used deep sequencing to identify BDNF (brain-derived neurotrophic factor)-induced miRNAs in cultured primary cortical mouse neurons. This revealed that the miR-212/132 cluster contained the miRNAs most responsive to BDNF treatment. This cluster was found to produce four miRNAs: miR-132, miR-132*, miR-212 and miR-212*. Using specific inhibitors, mouse models and promoter analysis we have shown that the regulation of the transcription of the miR-212/132 miRNA cluster and the miRNAs derived from it are regulated by the ERK1/2 (extracellular-signal-regulated kinase 1/2) pathway, via both MSK (mitogen and stress-activated kinase)-dependent and -independent mechanisms.

KW - Animals

KW - Base Sequence

KW - Benzamides

KW - Blotting, Northern

KW - Brain-Derived Neurotrophic Factor

KW - Cells, Cultured

KW - Cyclic AMP Response Element-Binding Protein

KW - Mice

KW - Mice, Inbred C57BL

KW - Mice, Knockout

KW - Mice, Transgenic

KW - MicroRNAs

KW - Mitogen-Activated Protein Kinase 1

KW - Mitogen-Activated Protein Kinase 3

KW - Molecular Sequence Data

KW - Neurons

KW - Nucleic Acid Amplification Techniques

KW - Phosphorylation

KW - Polymerase Chain Reaction

KW - Ribosomal Protein S6 Kinases, 90-kDa

KW - Sequence Homology, Nucleic Acid

U2 - 10.1042/BJ20100024

DO - 10.1042/BJ20100024

M3 - Article

C2 - 20307261

SN - 0264-6021

VL - 428

SP - 281

EP - 291

JO - Biochemical Journal

JF - Biochemical Journal

IS - 2

ER -

Regulation of the miR-212/132 locus by MSK1 and CREB in response to neurotrophins

Abstract / Description of output

Keywords / Materials (for Non-textual outputs)

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