Regulation of the production of granulocyte-macrophage colony-stimulating factor by macrophage-like tumour cell lines

D A Hume, K M Summers, D R Cohen, W Allan

Research output: Contribution to journalArticlepeer-review

Abstract

Macrophage tumour cell lines (PU5-1.8, P388D1) produced detectable granulocyte macrophage colony-stimulating (CSF-2) activity measured using a factor-dependent cell line FDC-P1. The production of CSF-2 was enhanced by endotoxin and inhibited by serum, and correlated inversely with [3H]TdR incorporation. mRNA isolated from PU5-1.8 or P388D1 cells initiated CSF-2 production when injected into Xenopus laevis oocytes. The specific activity in this assay was unaltered in mRNA isolated from endotoxin-treated cells. The results suggest that endotoxin acts at a post-transcriptional level.
Original languageEnglish
Pages (from-to)271-4
Number of pages4
JournalFEBS Letters
Volume180
Issue number2
DOIs
Publication statusPublished - 1985

Keywords

  • Animals
  • Cell Division/drug effects
  • Cell Line
  • Colony-Stimulating Factors/biosynthesis
  • Endotoxins/pharmacology
  • Female
  • Granulocytes/metabolism
  • Leukemia P388/metabolism
  • Leukemia, Experimental/metabolism
  • Leukemia, Myeloid/metabolism
  • Macrophages/metabolism
  • Mice
  • Xenopus laevis

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