Abstract
Macrophage tumour cell lines (PU5-1.8, P388D1) produced detectable granulocyte macrophage colony-stimulating (CSF-2) activity measured using a factor-dependent cell line FDC-P1. The production of CSF-2 was enhanced by endotoxin and inhibited by serum, and correlated inversely with [3H]TdR incorporation. mRNA isolated from PU5-1.8 or P388D1 cells initiated CSF-2 production when injected into Xenopus laevis oocytes. The specific activity in this assay was unaltered in mRNA isolated from endotoxin-treated cells. The results suggest that endotoxin acts at a post-transcriptional level.
| Original language | English |
|---|---|
| Pages (from-to) | 271-4 |
| Number of pages | 4 |
| Journal | FEBS Letters |
| Volume | 180 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 1985 |
Keywords / Materials (for Non-textual outputs)
- Animals
- Cell Division/drug effects
- Cell Line
- Colony-Stimulating Factors/biosynthesis
- Endotoxins/pharmacology
- Female
- Granulocytes/metabolism
- Leukemia P388/metabolism
- Leukemia, Experimental/metabolism
- Leukemia, Myeloid/metabolism
- Macrophages/metabolism
- Mice
- Xenopus laevis