Regulation of the proliferative activity of ovarian surface epithelial cells by follicular fluid

L. K. Doyle, F. X. Donadeu

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Despite critical roles of the ovarian surface epithelium (OSE) in ovulation and post-ovulatory wound repair, little is known about the physiological mechanism regulating OSE proliferation. A role of follicles and corpora lutea in locally regulating the proliferative activity of OSE has been suggested. In this study, the effects of follicular and luteal products on proliferation of cultured OSE cells were tested using cells obtained from seasonally anoestrous ewes. Follicular fluid but not luteal extracts induced OSE cell proliferation (2.5-fold relative to untreated controls; P<0.0001). The response of OSE cells was not affected by follicle size or previous charcoal-extraction of follicular fluid (P>0.1). Treatment with IGF-1 (2.2-fold: P<0.01), EGF (1.9-fold; P<0.01) and, to a lesser extent, FSH (P<0.05) also induced OSE cell proliferation. In contrast, oestradiol or progesterone did not induce cell proliferation or enhance the effects of FSH on proliferation (P>0.1). It was concluded that follicular fluid can directly stimulate ovine OSE cell proliferation and that this effect is attributable to non-steroidal mitogens. (C) 2008 Elsevier B.V. All rights reserved.
Original languageEnglish
Pages (from-to)443-8
Number of pages6
JournalAnim Reprod Sci
Issue number4
Publication statusPublished - Sep 2009


  • Animals
  • Brain Chemistry
  • Cell Line
  • Cell Survival
  • Hot Temperature
  • Humans
  • Mice
  • Prion Diseases/ pathology/ physiopathology
  • Species Specificity
  • Sterilization/ methods


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