Relationship between amino acid metabolism and bovine in vitro follicle activation and growth

Kenichiro Sakaguchi, Kohei Kawano, Yojiro Yanagawa, Seiji Katagiri, Evelyn E Telfer

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

The amino acid metabolism of bovine follicles during in vitro growth (IVG) was evaluated to identify potential indicators of health during culture. The bovine ovarian cortex was sliced, prepared as strips, and cultured for 6 days. Tissue samples were examined histologically before and after 6 days of culture, and the degree of follicle activation was classified as either high or low based on the number of growing secondary follicles present (high: 7~11; low: 0~1). In a separate experiment, secondary follicles (diameter range: 100~200 μm) were manually isolated and cultured, and their growth was monitored for 6 days. Cultured follicles were classified as growth or degenerate based on diameter change during culture (growth: +60.5~74.1 μm; degenerate: −28~15.2 μm). Free amino acids and their metabolites were measured in the spent culture medium from each group. In cultured ovarian cortical strips, the concentration of α-aminoadipic acid was significantly higher in the low activation group than in the high group (p < 0.05), while those of methionine, lysine, and arginine were higher in the high activation group. In cultured isolated secondary follicles, concentrations of methionine, tyrosine, histidine, and hydroxyproline were higher in the degenerate group (p ≤ 0.05). In conclusion, amino acid metabolism has the potential to serve as an indicator of primordial follicle activation and subsequent growth rate during bovine IVG.

Original languageEnglish
Article number1141
Number of pages16
JournalAnimals
Volume13
Issue number7
Early online date23 Mar 2023
DOIs
Publication statusPublished - 23 Mar 2023

Keywords / Materials (for Non-textual outputs)

  • amino acid
  • bovine
  • in vitro growth
  • primordial follicle
  • secondary follicle

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