Relationship of transcriptional markers to Leydig cell number in the mouse testis

Diane Rebourcet, Ana Monteiro, Lyndsey Cruickshanks, Nathan Jeffery, Sarah Smith, Laura Milne, Peter J O'Shaughnessy, Lee B Smith

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

OBJECTIVES: The current study aims to identify markers that would reflect the number of Leydig cells present in the testis, to help determine whether labour-intensive methods such as stereology are necessary. We used our well-characterised Sertoli cell ablation model in which we have empirically established the size of the Leydig cell population, to try to identify transcriptional biomarkers indicative of population size.

RESULTS: Following characterisation of the Leydig cell population after Sertoli cell ablation in neonatal life or adulthood, we identified Hsd3b1 transcript levels as a potential indicator of Leydig cell number with utility for informing decision-making on whether to engage in time-consuming stereological cell counting analysis.

Original languageEnglish
Article numbere0219524
JournalPLoS ONE
Issue number7
Publication statusPublished - 10 Jul 2019


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