Relative gene expression and quantification of IL-1 β simulated by Lipopolysaccharides (LPS) on human monocytes

Umer Chaudhry

Research output: Other contribution

Abstract / Description of output

Monocytes play key role in the onset of inflammation and immune response by
stimulating a diversity of cytokines includes IL, IFN and TNF. PAMPs normally
utilize to study the numerous pathways for the activation of innate immune are
Lipopolysaccharide (LPS) and polyinosinic-polycytidylic acid (poly I: C).
Lipopolysaccharide (LPS) is the major component of the cell wall of gram
negative bacteria can be identified by a receptor complex consist of CD14,
myeloid differentiation protein 2 and TLR-4. Polyinosinic polycytidylic acid
(polyI:C), and Lipopolysaccharide (LPS) were used to study IL-1 β response in
human monocytes. The results reveal a consistent increase in IL-1 β protein when
stimulated by LPS and Poly IC and determined by ELISA. While studying the
relative expression of IL-1 β by q-PCR when stimulated with LPS and Poly IC,
dramatic results were observed showing a cell exhaust resulting in an increase in
expression level uptill 2, and 3 hours and then a decline in expression when further
incubated.
Original languageEnglish
DOIs
Publication statusPublished - 2008

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