Projects per year
Protein-protein interactions govern most cellular pathways and processes, and multiplete chnologies have emerged to systematically map them. Assessing the error of interaction networks has been a challenge. Crosslinking mass spectrometry is currently widening its scope from structural analyses of purified multi-protein complexes towards systems-wide analyses of protein-protein interactions (PPIs). Using a carefully controlled large-scale analysis of Escherichia coli cell lysate, we demonstrate that false-discovery rates (FDR) for PPIs identified by crosslinking mass spectrometry can be reliably estimated. We present an interaction network comprising 590 PPIs at 1% decoy-based PPI-FDR. The structural information included in this network localises the binding site of the hitherto uncharacterised protein YacL to near the DNA exit tunnel on the RNA polymerase.
|Publication status||Accepted/In press - 28 Apr 2021|
`Core Funding for the Wellcome Trust Centre for Cell Biology¿, Research Enrichment, Public Engagement
1/12/18 → 1/06/22
1/06/14 → 31/05/21