TY - JOUR
T1 - Relief of Biofilm Hypoxia: A Synergistic Approach with Cyanobacteria and Chlorin e6-Loaded Nanoparticles
AU - Kashif, Saima
AU - Roberts, Sam
AU - Gopal, Ashna
AU - Schiavon Osorio, Alejandra A.
AU - Nenninger, Anja
AU - Yan, Li
AU - McCormick, Alistair J.
AU - Chen, Michael
N1 - We thank Dr. Sander Granneman in the School of Biological Sciences at The University of Edinburgh for providing the bacterial strain used in this research. We acknowledge the use of the Cryo FIB/SEM bought with the EPSRC grant EP/P030564/1 and Fraser Laidlaw for help with image acquisition. AJM and AN acknowledge funding support from the UK Research and Innovation Biotechnology and Biological Sciences Research Council (UKRI-BBSRC) (BB/ S020128/1). This research was funded by Scottish Funding Council- -Global Challenges Research Fund.
PY - 2024/8/12
Y1 - 2024/8/12
N2 - Photodynamic therapy (PDT) involves the use of photosensitizers, oxygen, and light to generate reactive oxygen species (ROS) for the effective destruction of cancer cells or bacteria. However, in biofilm bacterial infections, the presence of hypoxia significantly reduces the efficacy of PDT. To address this issue, we have developed a synergistic approach wherein Synechococcus elongatus (PCC 7942), cyanobacteria are loaded into a sodium alginate hydrogel, and chlorin e6 (Ce6) is conjugated with mesoporous silica nanoparticles (Ce6-MSNs). Cyanobacteria are photosynthetic organisms capable of releasing oxygen when exposed to 660 nm light. This property facilitates the effective production of ROS by Ce6-MSNs, even in hypoxic conditions, potentially enabling more effective PDT against MRSA planktonic bacteria and biofilm. Our results demonstrate that this system can maintain oxygen generation capacity for the test period of 30 days, thereby enhancing ROS production under hypoxic conditions. In summary, this innovative system combines cyanobacteria's photosynthetic oxygen generation with Ce6-MSNs' ROS production capabilities to address the challenge of hypoxia in biofilm infections. The promising results suggest its potential for improving the efficacy of PDT against bacterial infections, including drug-resistant strains such as MRSA.
AB - Photodynamic therapy (PDT) involves the use of photosensitizers, oxygen, and light to generate reactive oxygen species (ROS) for the effective destruction of cancer cells or bacteria. However, in biofilm bacterial infections, the presence of hypoxia significantly reduces the efficacy of PDT. To address this issue, we have developed a synergistic approach wherein Synechococcus elongatus (PCC 7942), cyanobacteria are loaded into a sodium alginate hydrogel, and chlorin e6 (Ce6) is conjugated with mesoporous silica nanoparticles (Ce6-MSNs). Cyanobacteria are photosynthetic organisms capable of releasing oxygen when exposed to 660 nm light. This property facilitates the effective production of ROS by Ce6-MSNs, even in hypoxic conditions, potentially enabling more effective PDT against MRSA planktonic bacteria and biofilm. Our results demonstrate that this system can maintain oxygen generation capacity for the test period of 30 days, thereby enhancing ROS production under hypoxic conditions. In summary, this innovative system combines cyanobacteria's photosynthetic oxygen generation with Ce6-MSNs' ROS production capabilities to address the challenge of hypoxia in biofilm infections. The promising results suggest its potential for improving the efficacy of PDT against bacterial infections, including drug-resistant strains such as MRSA.
KW - Photodynamic therapy
KW - cyanobacteria
KW - antibacterial
KW - hypoxia
KW - biofilm
U2 - 10.1007/s44174-024-00219-8
DO - 10.1007/s44174-024-00219-8
M3 - Article
SN - 2731-4820
JO - Biomedical Materials and Devices
JF - Biomedical Materials and Devices
ER -