Renal Ischaemia Reperfusion Injury: A Mouse Model of Injury and Regeneration

Emily E. Hesketh*, Alicja Czopek, Michael Clay, Gary Borthwick, David Ferenbach, David Kluth, Jeremy Hughes

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Renal ischaemia reperfusion injury (IRI) is a common cause of acute kidney injury (AKI) in patients and occlusion of renal blood flow is unavoidable during renal transplantation. Experimental models that accurately and reproducibly recapitulate renal IRI are crucial in dissecting the pathophysiology of AKI and the development of novel therapeutic agents. Presented here is a mouse model of renal IRI that results in reproducible AKI. This is achieved by a midline laparotomy approach for the surgery with one incision allowing both a right nephrectomy that provides control tissue and clamping of the left renal pedicle to induce ischaemia of the left kidney. By careful monitoring of the clamp position and body temperature during the period of ischaemia this model achieves reproducible functional and structural injury. Mice sacrificed 24 hr following surgery demonstrate loss of renal function with elevation of the serum or plasma creatinine level as well as structural kidney damage with acute tubular necrosis evident. Renal function improves and the acute tissue injury resolves during the course of 7 days following renal IRI such that this model may be used to study renal regeneration. This model of renal IRI has been utilized to study the molecular and cellular pathophysiology of AKI as well as analysis of the subsequent renal regeneration.

Original languageEnglish
Article number51816
Number of pages8
JournalJournal of Visualized Experiments (JoVE)
Issue number88
DOIs
Publication statusPublished - Jun 2014

Keywords

  • Medicine
  • Issue 88
  • Murine
  • Acute Kidney Injury
  • Ischaemia
  • Reperfusion
  • Nephrectomy
  • Regeneration
  • Laparotomy
  • ACUTE KIDNEY INJURY
  • ISCHEMIA/REPERFUSION INJURY
  • HEME OXYGENASE-1
  • REPAIR
  • CELLS
  • MACROPHAGES
  • MICE
  • HEMEOXYGENASE-1

Cite this