Repair of osteochondral defects in the minipig model by OPF hydrogel loaded with adipose-derived mesenchymal stem cells

L. De Girolamo; S. Niada; E. Arrigoni; A. Di Giancamillo; C. Domeneghini; M. Dadsetan; M.J. Yaszemski; D. Gastaldi; P. Vena; M. Taffetani; A. Zerbi; V. Sansone; G.M. Peretti; A.T. Brini

doi:10.2217/rme.14.77

Repair of osteochondral defects in the minipig model by OPF hydrogel loaded with adipose-derived mesenchymal stem cells

L. De Girolamo, S. Niada, E. Arrigoni, A. Di Giancamillo, C. Domeneghini, M. Dadsetan, M.J. Yaszemski, D. Gastaldi, P. Vena, M. Taffetani, A. Zerbi, V. Sansone, G.M. Peretti, A.T. Brini

School of Engineering

Research output: Contribution to journal › Article › peer-review

Abstract

Aim: Critical knee osteochondral defects in seven adult minipigs were treated with oligo(polyethylene glycol)fumarate (OPF) hydrogel combined with autologous or human adipose-derived stem cells (ASCs), and evaluated after 6 months. Methods: Four defects were made on the peripheral part of right trochleas (n = 28), and treated with OPF scaffold alone or pre-seeded with ASCs.

Results: A better quality cartilage tissue characterized by improved biomechanical properties and higher collagen type II expression was observed in the defects treated by autologous or human ASC-loaded OPF; similarly this approach induced the regeneration of more mature bone with upregulation of collagen type I expression.

Conclusion: This study provides the evidence that both porcine and human adipose-derived stem cells associated to OPF hydrogel allow improving osteochondral defect regeneration in a minipig model.

Original language	Undefined/Unknown
Journal	Regenerative medicine
Volume	10
Issue number	2
DOIs	https://doi.org/10.2217/rme.14.77
Publication status	Published - 2 Apr 2015

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De Girolamo, L., Niada, S., Arrigoni, E., Di Giancamillo, A., Domeneghini, C., Dadsetan, M., Yaszemski, M. J., Gastaldi, D., Vena, P., Taffetani, M., Zerbi, A., Sansone, V., Peretti, G. M., & Brini, A. T. (2015). Repair of osteochondral defects in the minipig model by OPF hydrogel loaded with adipose-derived mesenchymal stem cells. Regenerative medicine, 10(2). https://doi.org/10.2217/rme.14.77

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abstract = "Aim: Critical knee osteochondral defects in seven adult minipigs were treated with oligo(polyethylene glycol)fumarate (OPF) hydrogel combined with autologous or human adipose-derived stem cells (ASCs), and evaluated after 6 months. Methods: Four defects were made on the peripheral part of right trochleas (n = 28), and treated with OPF scaffold alone or pre-seeded with ASCs.Results: A better quality cartilage tissue characterized by improved biomechanical properties and higher collagen type II expression was observed in the defects treated by autologous or human ASC-loaded OPF; similarly this approach induced the regeneration of more mature bone with upregulation of collagen type I expression. Conclusion: This study provides the evidence that both porcine and human adipose-derived stem cells associated to OPF hydrogel allow improving osteochondral defect regeneration in a minipig model.",

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De Girolamo, L, Niada, S, Arrigoni, E, Di Giancamillo, A, Domeneghini, C, Dadsetan, M, Yaszemski, MJ, Gastaldi, D, Vena, P, Taffetani, M, Zerbi, A, Sansone, V, Peretti, GM & Brini, AT 2015, 'Repair of osteochondral defects in the minipig model by OPF hydrogel loaded with adipose-derived mesenchymal stem cells', Regenerative medicine, vol. 10, no. 2. https://doi.org/10.2217/rme.14.77

TY - JOUR

T1 - Repair of osteochondral defects in the minipig model by OPF hydrogel loaded with adipose-derived mesenchymal stem cells

AU - De Girolamo, L.

AU - Niada, S.

AU - Arrigoni, E.

AU - Di Giancamillo, A.

AU - Domeneghini, C.

AU - Dadsetan, M.

AU - Yaszemski, M.J.

AU - Gastaldi, D.

AU - Vena, P.

AU - Taffetani, M.

AU - Zerbi, A.

AU - Sansone, V.

AU - Peretti, G.M.

AU - Brini, A.T.

PY - 2015/4/2

Y1 - 2015/4/2

N2 - Aim: Critical knee osteochondral defects in seven adult minipigs were treated with oligo(polyethylene glycol)fumarate (OPF) hydrogel combined with autologous or human adipose-derived stem cells (ASCs), and evaluated after 6 months. Methods: Four defects were made on the peripheral part of right trochleas (n = 28), and treated with OPF scaffold alone or pre-seeded with ASCs.Results: A better quality cartilage tissue characterized by improved biomechanical properties and higher collagen type II expression was observed in the defects treated by autologous or human ASC-loaded OPF; similarly this approach induced the regeneration of more mature bone with upregulation of collagen type I expression. Conclusion: This study provides the evidence that both porcine and human adipose-derived stem cells associated to OPF hydrogel allow improving osteochondral defect regeneration in a minipig model.

AB - Aim: Critical knee osteochondral defects in seven adult minipigs were treated with oligo(polyethylene glycol)fumarate (OPF) hydrogel combined with autologous or human adipose-derived stem cells (ASCs), and evaluated after 6 months. Methods: Four defects were made on the peripheral part of right trochleas (n = 28), and treated with OPF scaffold alone or pre-seeded with ASCs.Results: A better quality cartilage tissue characterized by improved biomechanical properties and higher collagen type II expression was observed in the defects treated by autologous or human ASC-loaded OPF; similarly this approach induced the regeneration of more mature bone with upregulation of collagen type I expression. Conclusion: This study provides the evidence that both porcine and human adipose-derived stem cells associated to OPF hydrogel allow improving osteochondral defect regeneration in a minipig model.

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U2 - 10.2217/rme.14.77

DO - 10.2217/rme.14.77

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SN - 1746-0751

VL - 10

JO - Regenerative medicine

JF - Regenerative medicine

IS - 2

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Repair of osteochondral defects in the minipig model by OPF hydrogel loaded with adipose-derived mesenchymal stem cells

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