Repo-Man Coordinates Chromosomal Reorganization with Nuclear Envelope Reassembly during Mitotic Exit

Paola Vagnarelli, Susana Ribeiro, Lau Sennels, Luis Sanchez-Pulido, Flavia de Lima Alves, Toon Verheyen, David A. Kelly, Chris P. Ponting, Juri Rappsilber, William C. Earnshaw

Research output: Contribution to journalArticlepeer-review


Repo-Man targets protein phosphatase 1 gamma (PP1 gamma) to chromatin at anaphase onset and regulates chromosome structure during mitotic exit. Here, we show that a Repo-Man:PP1 complex forms in anaphase following dephosphorylation of Repo-Man. Upon activation, the complex localizes to chromosomes and causes the dephosphorylation of histone H3 (Thr3, Ser10, and Ser28). In anaphase, Repo-Man has both catalytic and structural functions that are mediated by two separate domains. A C-terminal domain localizes Repo-Man to bulk chromatin in early anaphase. There, it targets PP1 for the dephosphorylation of histone H3 and possibly other chromosomal substrates. An N-terminal domain localizes Repo-Man to the chromosome periphery later in anaphase. There, it is responsible for the recruitment of nuclear components such as Importin and Nup153 in a PP1-independent manner. These observations identify Repo-Man as a key factor that coordinates chromatin remodeling and early events of nuclear envelope reformation during mitotic exit.

Original languageEnglish
Pages (from-to)328-342
Number of pages15
JournalDevelopmental Cell
Issue number2
Publication statusPublished - 16 Aug 2011


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