Repo-Man/PP1 regulates heterochromatin formation in interphase

Ines J de Castro; James Budzak; Maria L Giacinto; Lorena Ligammari; Ezgi Gokhan; Christos Spanos; Daniela Moralli; Christine Richardson; Jose de las Heras; Silvia Salatino; Eric Schirmer; Kathrine Ullman; Wendy Bickmore; Catherine Green; Juri Rappsilber; Sarah Lamble; Martin W Goldberg; Veronica Vinciotti; P. Vagnarelli

doi:10.1038/ncomms14048

Repo-Man/PP1 regulates heterochromatin formation in interphase

Ines J de Castro, James Budzak, Maria L Giacinto, Lorena Ligammari, Ezgi Gokhan, Christos Spanos, Daniela Moralli, Christine Richardson, Jose de las Heras, Silvia Salatino, Eric Schirmer, Kathrine Ullman, Wendy Bickmore, Catherine Green, Juri Rappsilber, Sarah Lamble, Martin W Goldberg, Veronica Vinciotti, P. Vagnarelli

Research output: Contribution to journal › Article › peer-review

Abstract

Repo-Man is a protein phosphatase 1 (PP1) targeting subunit that regulates mitotic progression and chromatin remodelling. After mitosis, Repo-Man/PP1 remains associated with chromatin but its function in interphase is not known. Here we show that Repo-Man, via Nup153, is enriched on condensed chromatin at the nuclear periphery and at the edge of the nucleopore basket. Repo-Man/PP1 regulates the formation of heterochromatin, dephosphorylates H3S28 and it is necessary and sufficient for heterochromatin protein 1 binding and H3K27me3 recruitment. Using a novel proteogenomic approach, we show that Repo-Man is enriched at subtelomeric regions together with H2AZ and H3.3 and that depletion of Repo-Man alters the peripheral localization of a subset of these regions and alleviates repression of some polycomb telomeric genes. This study shows a role for a mitotic phosphatase in the regulation of the epigenetic landscape and gene expression in interphase.

Original language	English
Article number	14048
Number of pages	15
Journal	Nature Communications
Volume	8
Early online date	16 Jan 2017
DOIs	https://doi.org/10.1038/ncomms14048
Publication status	Published - 2017

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Repo_Man PP1 regulates heterochromatin formation 1 in interphaseAccepted author manuscript, 2.79 MBLicence: Creative Commons: Attribution (CC-BY)
Repo_Man_PP1 regulates heterochromatin
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Final published version, 3.17 MBLicence: Creative Commons: Attribution (CC-BY)

The role of spatial nuclear organisation in genome function
Bickmore, W.
1/04/12 → 31/03/18
Project: Research
HGU Core Award Apr 12 - MAr 18
Hastie, N., Adams, I., Baldock, R., Bickmore, W., Caceres, J., Dorin, J., FitzPatrick, D., Haley, C., Hill, B., Jackson, I., Jackson, A., Kudla, G., Meehan, R. & Patton, E.
MRC
1/04/12 → 31/03/18
Project: Research

Cite this

de Castro, I. J., Budzak, J., Giacinto, M. L., Ligammari, L., Gokhan, E., Spanos, C., Moralli, D., Richardson, C., de las Heras, J., Salatino, S., Schirmer, E., Ullman, K., Bickmore, W., Green, C., Rappsilber, J., Lamble, S., Goldberg, M. W., Vinciotti, V., & Vagnarelli, P. (2017). Repo-Man/PP1 regulates heterochromatin formation in interphase. Nature Communications, 8, Article 14048. https://doi.org/10.1038/ncomms14048

@article{f99a605ce42946338e4bef773f433832,

title = "Repo-Man/PP1 regulates heterochromatin formation in interphase",

abstract = "Repo-Man is a protein phosphatase 1 (PP1) targeting subunit that regulates mitotic progression and chromatin remodelling. After mitosis, Repo-Man/PP1 remains associated with chromatin but its function in interphase is not known. Here we show that Repo-Man, via Nup153, is enriched on condensed chromatin at the nuclear periphery and at the edge of the nucleopore basket. Repo-Man/PP1 regulates the formation of heterochromatin, dephosphorylates H3S28 and it is necessary and sufficient for heterochromatin protein 1 binding and H3K27me3 recruitment. Using a novel proteogenomic approach, we show that Repo-Man is enriched at subtelomeric regions together with H2AZ and H3.3 and that depletion of Repo-Man alters the peripheral localization of a subset of these regions and alleviates repression of some polycomb telomeric genes. This study shows a role for a mitotic phosphatase in the regulation of the epigenetic landscape and gene expression in interphase.",

author = "{de Castro}, {Ines J} and James Budzak and Giacinto, {Maria L} and Lorena Ligammari and Ezgi Gokhan and Christos Spanos and Daniela Moralli and Christine Richardson and {de las Heras}, Jose and Silvia Salatino and Eric Schirmer and Kathrine Ullman and Wendy Bickmore and Catherine Green and Juri Rappsilber and Sarah Lamble and Goldberg, {Martin W} and Veronica Vinciotti and P. Vagnarelli",

year = "2017",

doi = "10.1038/ncomms14048",

language = "English",

volume = "8",

journal = "Nature Communications",

issn = "2041-1723",

publisher = "Nature Publishing Group",

}

de Castro, IJ, Budzak, J, Giacinto, ML, Ligammari, L, Gokhan, E, Spanos, C, Moralli, D, Richardson, C, de las Heras, J, Salatino, S, Schirmer, E, Ullman, K, Bickmore, W, Green, C, Rappsilber, J, Lamble, S, Goldberg, MW, Vinciotti, V & Vagnarelli, P 2017, 'Repo-Man/PP1 regulates heterochromatin formation in interphase', Nature Communications, vol. 8, 14048. https://doi.org/10.1038/ncomms14048

TY - JOUR

T1 - Repo-Man/PP1 regulates heterochromatin formation in interphase

AU - de Castro, Ines J

AU - Budzak, James

AU - Giacinto, Maria L

AU - Ligammari, Lorena

AU - Gokhan, Ezgi

AU - Spanos, Christos

AU - Moralli, Daniela

AU - Richardson, Christine

AU - de las Heras, Jose

AU - Salatino, Silvia

AU - Schirmer, Eric

AU - Ullman, Kathrine

AU - Bickmore, Wendy

AU - Green, Catherine

AU - Rappsilber, Juri

AU - Lamble, Sarah

AU - Goldberg, Martin W

AU - Vinciotti, Veronica

AU - Vagnarelli, P.

PY - 2017

Y1 - 2017

N2 - Repo-Man is a protein phosphatase 1 (PP1) targeting subunit that regulates mitotic progression and chromatin remodelling. After mitosis, Repo-Man/PP1 remains associated with chromatin but its function in interphase is not known. Here we show that Repo-Man, via Nup153, is enriched on condensed chromatin at the nuclear periphery and at the edge of the nucleopore basket. Repo-Man/PP1 regulates the formation of heterochromatin, dephosphorylates H3S28 and it is necessary and sufficient for heterochromatin protein 1 binding and H3K27me3 recruitment. Using a novel proteogenomic approach, we show that Repo-Man is enriched at subtelomeric regions together with H2AZ and H3.3 and that depletion of Repo-Man alters the peripheral localization of a subset of these regions and alleviates repression of some polycomb telomeric genes. This study shows a role for a mitotic phosphatase in the regulation of the epigenetic landscape and gene expression in interphase.

AB - Repo-Man is a protein phosphatase 1 (PP1) targeting subunit that regulates mitotic progression and chromatin remodelling. After mitosis, Repo-Man/PP1 remains associated with chromatin but its function in interphase is not known. Here we show that Repo-Man, via Nup153, is enriched on condensed chromatin at the nuclear periphery and at the edge of the nucleopore basket. Repo-Man/PP1 regulates the formation of heterochromatin, dephosphorylates H3S28 and it is necessary and sufficient for heterochromatin protein 1 binding and H3K27me3 recruitment. Using a novel proteogenomic approach, we show that Repo-Man is enriched at subtelomeric regions together with H2AZ and H3.3 and that depletion of Repo-Man alters the peripheral localization of a subset of these regions and alleviates repression of some polycomb telomeric genes. This study shows a role for a mitotic phosphatase in the regulation of the epigenetic landscape and gene expression in interphase.

U2 - 10.1038/ncomms14048

DO - 10.1038/ncomms14048

M3 - Article

SN - 2041-1723

VL - 8

JO - Nature Communications

JF - Nature Communications

M1 - 14048

ER -

Repo-Man/PP1 regulates heterochromatin formation in interphase

Abstract

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The role of spatial nuclear organisation in genome function

HGU Core Award Apr 12 - MAr 18

Cite this