Reproducibility of nuclear morphometry parameters from cytologic smears of canine cutaneous mast cell tumors--intra- and interobserver variability

Daniella de F L Barbosa; Mariana D Paraventi; Ricardo de F Strefezzi

doi:10.1111/vcp.12167

Reproducibility of nuclear morphometry parameters from cytologic smears of canine cutaneous mast cell tumors--intra- and interobserver variability

Daniella de F L Barbosa, Mariana D Paraventi, Ricardo de F Strefezzi

Royal (Dick) School of Veterinary Studies

Research output: Contribution to journal › Article › peer-review

Abstract

BACKGROUND: Qualitative and/or semiquantitative histopathologic grading has been the method of choice to predict the biologic behavior of mast cell tumors (MCT). Recently, computer-assisted nuclear morphometric analysis of histologic or cytologic specimens has been shown to correlate with histopathologic grading of MCT. However, it is still not clear what the reproducibility and variability of these computer-assisted measurements are.

OBJECTIVES: The objective of this study was to estimate the intra- and interobserver variability in computer-assisted morphometric analysis of nuclear parameters in fine-needle cytologic smears of canine cutaneous MCT.

MATERIALS AND METHODS: Measurements were performed by 2 observers with basic training on the recognition of neoplastic cells and operating the image analysis software. The analyses were performed twice at 6 months apart.

RESULTS: No significant effects were detected for observer, time, and the interaction observer-time.

CONCLUSIONS: Nuclear morphometric analysis of mast cell tumors using cytologic smears is a reproducible method for grading of MCT.

Original language	English
Pages (from-to)	469-72
Number of pages	4
Journal	Veterinary Clinical Pathology: An International Journal of Laboratory Medicine
Volume	43
Issue number	3
DOIs	https://doi.org/10.1111/vcp.12167
Publication status	E-pub ahead of print - 27 Jun 2014

Keywords

Animals
Biopsy, Fine-Needle/veterinary
Cell Nucleus/pathology
Dog Diseases/pathology
Dogs
Female
Image Processing, Computer-Assisted
Male
Mast Cells/pathology
Mastocytoma, Skin/pathology
Neoplasm Grading/veterinary
Observer Variation
Reproducibility of Results
Software

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10.1111/vcp.12167

Cite this

@article{004ad8f1ded04c1a9b6b1a3ff82d925e,

title = "Reproducibility of nuclear morphometry parameters from cytologic smears of canine cutaneous mast cell tumors--intra- and interobserver variability",

abstract = "BACKGROUND: Qualitative and/or semiquantitative histopathologic grading has been the method of choice to predict the biologic behavior of mast cell tumors (MCT). Recently, computer-assisted nuclear morphometric analysis of histologic or cytologic specimens has been shown to correlate with histopathologic grading of MCT. However, it is still not clear what the reproducibility and variability of these computer-assisted measurements are.OBJECTIVES: The objective of this study was to estimate the intra- and interobserver variability in computer-assisted morphometric analysis of nuclear parameters in fine-needle cytologic smears of canine cutaneous MCT.MATERIALS AND METHODS: Measurements were performed by 2 observers with basic training on the recognition of neoplastic cells and operating the image analysis software. The analyses were performed twice at 6 months apart.RESULTS: No significant effects were detected for observer, time, and the interaction observer-time.CONCLUSIONS: Nuclear morphometric analysis of mast cell tumors using cytologic smears is a reproducible method for grading of MCT.",

keywords = "Animals, Biopsy, Fine-Needle/veterinary, Cell Nucleus/pathology, Dog Diseases/pathology, Dogs, Female, Image Processing, Computer-Assisted, Male, Mast Cells/pathology, Mastocytoma, Skin/pathology, Neoplasm Grading/veterinary, Observer Variation, Reproducibility of Results, Software",

author = "Barbosa, {Daniella de F L} and Paraventi, {Mariana D} and Strefezzi, {Ricardo de F}",

note = "{\textcopyright} 2014 American Society for Veterinary Clinical Pathology and European Society for Veterinary Clinical Pathology.",

year = "2014",

month = jun,

day = "27",

doi = "10.1111/vcp.12167",

language = "English",

volume = "43",

pages = "469--72",

journal = "Veterinary Clinical Pathology: An International Journal of Laboratory Medicine",

issn = "0275-6382",

publisher = "Wiley",

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Reproducibility of nuclear morphometry parameters from cytologic smears of canine cutaneous mast cell tumors--intra- and interobserver variability. / Barbosa, Daniella de F L; Paraventi, Mariana D; Strefezzi, Ricardo de F.

In: Veterinary Clinical Pathology: An International Journal of Laboratory Medicine, Vol. 43, No. 3, 27.06.2014, p. 469-72.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Reproducibility of nuclear morphometry parameters from cytologic smears of canine cutaneous mast cell tumors--intra- and interobserver variability

AU - Barbosa, Daniella de F L

AU - Paraventi, Mariana D

AU - Strefezzi, Ricardo de F

PY - 2014/6/27

Y1 - 2014/6/27

N2 - BACKGROUND: Qualitative and/or semiquantitative histopathologic grading has been the method of choice to predict the biologic behavior of mast cell tumors (MCT). Recently, computer-assisted nuclear morphometric analysis of histologic or cytologic specimens has been shown to correlate with histopathologic grading of MCT. However, it is still not clear what the reproducibility and variability of these computer-assisted measurements are.OBJECTIVES: The objective of this study was to estimate the intra- and interobserver variability in computer-assisted morphometric analysis of nuclear parameters in fine-needle cytologic smears of canine cutaneous MCT.MATERIALS AND METHODS: Measurements were performed by 2 observers with basic training on the recognition of neoplastic cells and operating the image analysis software. The analyses were performed twice at 6 months apart.RESULTS: No significant effects were detected for observer, time, and the interaction observer-time.CONCLUSIONS: Nuclear morphometric analysis of mast cell tumors using cytologic smears is a reproducible method for grading of MCT.

AB - BACKGROUND: Qualitative and/or semiquantitative histopathologic grading has been the method of choice to predict the biologic behavior of mast cell tumors (MCT). Recently, computer-assisted nuclear morphometric analysis of histologic or cytologic specimens has been shown to correlate with histopathologic grading of MCT. However, it is still not clear what the reproducibility and variability of these computer-assisted measurements are.OBJECTIVES: The objective of this study was to estimate the intra- and interobserver variability in computer-assisted morphometric analysis of nuclear parameters in fine-needle cytologic smears of canine cutaneous MCT.MATERIALS AND METHODS: Measurements were performed by 2 observers with basic training on the recognition of neoplastic cells and operating the image analysis software. The analyses were performed twice at 6 months apart.RESULTS: No significant effects were detected for observer, time, and the interaction observer-time.CONCLUSIONS: Nuclear morphometric analysis of mast cell tumors using cytologic smears is a reproducible method for grading of MCT.

KW - Animals

KW - Biopsy, Fine-Needle/veterinary

KW - Cell Nucleus/pathology

KW - Dog Diseases/pathology

KW - Dogs

KW - Female

KW - Image Processing, Computer-Assisted

KW - Male

KW - Mast Cells/pathology

KW - Mastocytoma, Skin/pathology

KW - Neoplasm Grading/veterinary

KW - Observer Variation

KW - Reproducibility of Results

KW - Software

U2 - 10.1111/vcp.12167

DO - 10.1111/vcp.12167

M3 - Article

C2 - 24976414

VL - 43

SP - 469

EP - 472

JO - Veterinary Clinical Pathology: An International Journal of Laboratory Medicine

JF - Veterinary Clinical Pathology: An International Journal of Laboratory Medicine

SN - 0275-6382

IS - 3

ER -

Reproducibility of nuclear morphometry parameters from cytologic smears of canine cutaneous mast cell tumors--intra- and interobserver variability

Abstract

Keywords

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