Retinoic acid receptors initiate induction of the cytomegalovirus enhancer in embryonal cells

P Ghazal; C DeMattei; E Giulietti; S A Kliewer; K Umesono; R M Evans

doi:10.1073/pnas.89.16.7630

Retinoic acid receptors initiate induction of the cytomegalovirus enhancer in embryonal cells

P Ghazal, C DeMattei, E Giulietti, S A Kliewer, K Umesono, R M Evans

Deanery of Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

Abstract / Description of output

Reactivation of latent virus is believed to result from a signal transduction event that induces immediate-early (IE) gene transcription. Evidence is presented that the major IE promoter (MIEP) of human cytomegalovirus (hCMV) is activated by physiological levels of retinoic acid (RA) in human embryonal carcinoma cells. Mutagenesis experiments localized in the MIEP enhancer, a retinoic acid-responsive element composed of a direct repeat separated by five nucleotides. Protein-DNA binding experiments revealed that this element functions as a specific target site for the direct interaction of nuclear receptor proteins for RA. These findings implicate the biologically active derivative of vitamin A (RA) as a potential modulator of hCMV pathogenesis in infants and immunocompromised adults.

Original language	English
Pages (from-to)	7630-4
Number of pages	5
Journal	Proceedings of the National Academy of Sciences (PNAS)
Volume	89
Issue number	16
DOIs	https://doi.org/10.1073/pnas.89.16.7630
Publication status	Published - 1992

Keywords / Materials (for Non-textual outputs)

Animals
Antigens, Viral
Base Sequence
Carrier Proteins
Cell Line
Cloning, Molecular
Cytomegalovirus
Embryo, Mammalian
Enhancer Elements, Genetic
Gene Expression Regulation, Viral
Humans
Immediate-Early Proteins
Molecular Sequence Data
Mutagenesis, Site-Directed
Promoter Regions, Genetic
Receptors, Retinoic Acid
Recombinant Proteins
Signal Transduction
Transcription, Genetic
Transcriptional Activation
Viral Matrix Proteins
Virus Activation

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10.1073/pnas.89.16.7630

http://www.pnas.org/content/89/16/7630

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title = "Retinoic acid receptors initiate induction of the cytomegalovirus enhancer in embryonal cells",

abstract = "Reactivation of latent virus is believed to result from a signal transduction event that induces immediate-early (IE) gene transcription. Evidence is presented that the major IE promoter (MIEP) of human cytomegalovirus (hCMV) is activated by physiological levels of retinoic acid (RA) in human embryonal carcinoma cells. Mutagenesis experiments localized in the MIEP enhancer, a retinoic acid-responsive element composed of a direct repeat separated by five nucleotides. Protein-DNA binding experiments revealed that this element functions as a specific target site for the direct interaction of nuclear receptor proteins for RA. These findings implicate the biologically active derivative of vitamin A (RA) as a potential modulator of hCMV pathogenesis in infants and immunocompromised adults.",

keywords = "Animals, Antigens, Viral, Base Sequence, Carrier Proteins, Cell Line, Cloning, Molecular, Cytomegalovirus, Embryo, Mammalian, Enhancer Elements, Genetic, Gene Expression Regulation, Viral, Humans, Immediate-Early Proteins, Molecular Sequence Data, Mutagenesis, Site-Directed, Promoter Regions, Genetic, Receptors, Retinoic Acid, Recombinant Proteins, Signal Transduction, Transcription, Genetic, Transcriptional Activation, Viral Matrix Proteins, Virus Activation",

author = "P Ghazal and C DeMattei and E Giulietti and Kliewer, {S A} and K Umesono and Evans, {R M}",

year = "1992",

doi = "10.1073/pnas.89.16.7630",

language = "English",

volume = "89",

pages = "7630--4",

journal = "Proceedings of the National Academy of Sciences (PNAS)",

issn = "0027-8424",

publisher = "National Academy of Sciences",

number = "16",

}

TY - JOUR

T1 - Retinoic acid receptors initiate induction of the cytomegalovirus enhancer in embryonal cells

AU - Ghazal, P

AU - DeMattei, C

AU - Giulietti, E

AU - Kliewer, S A

AU - Umesono, K

AU - Evans, R M

PY - 1992

Y1 - 1992

N2 - Reactivation of latent virus is believed to result from a signal transduction event that induces immediate-early (IE) gene transcription. Evidence is presented that the major IE promoter (MIEP) of human cytomegalovirus (hCMV) is activated by physiological levels of retinoic acid (RA) in human embryonal carcinoma cells. Mutagenesis experiments localized in the MIEP enhancer, a retinoic acid-responsive element composed of a direct repeat separated by five nucleotides. Protein-DNA binding experiments revealed that this element functions as a specific target site for the direct interaction of nuclear receptor proteins for RA. These findings implicate the biologically active derivative of vitamin A (RA) as a potential modulator of hCMV pathogenesis in infants and immunocompromised adults.

AB - Reactivation of latent virus is believed to result from a signal transduction event that induces immediate-early (IE) gene transcription. Evidence is presented that the major IE promoter (MIEP) of human cytomegalovirus (hCMV) is activated by physiological levels of retinoic acid (RA) in human embryonal carcinoma cells. Mutagenesis experiments localized in the MIEP enhancer, a retinoic acid-responsive element composed of a direct repeat separated by five nucleotides. Protein-DNA binding experiments revealed that this element functions as a specific target site for the direct interaction of nuclear receptor proteins for RA. These findings implicate the biologically active derivative of vitamin A (RA) as a potential modulator of hCMV pathogenesis in infants and immunocompromised adults.

KW - Animals

KW - Antigens, Viral

KW - Base Sequence

KW - Carrier Proteins

KW - Cell Line

KW - Cloning, Molecular

KW - Cytomegalovirus

KW - Embryo, Mammalian

KW - Enhancer Elements, Genetic

KW - Gene Expression Regulation, Viral

KW - Humans

KW - Immediate-Early Proteins

KW - Molecular Sequence Data

KW - Mutagenesis, Site-Directed

KW - Promoter Regions, Genetic

KW - Receptors, Retinoic Acid

KW - Recombinant Proteins

KW - Signal Transduction

KW - Transcription, Genetic

KW - Transcriptional Activation

KW - Viral Matrix Proteins

KW - Virus Activation

U2 - 10.1073/pnas.89.16.7630

DO - 10.1073/pnas.89.16.7630

M3 - Article

C2 - 1323848

SN - 0027-8424

VL - 89

SP - 7630

EP - 7634

JO - Proceedings of the National Academy of Sciences (PNAS)

JF - Proceedings of the National Academy of Sciences (PNAS)

IS - 16

ER -

Retinoic acid receptors initiate induction of the cytomegalovirus enhancer in embryonal cells

Abstract / Description of output

Keywords / Materials (for Non-textual outputs)

Access to Document

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Cite this