Retrospective application of transposon-directed insertion site sequencing to a library of signature-tagged mini-Tn5Km2 mutants of Escherichia coli O157:H7 screened in cattle

Sabine E. Eckert, Francis Dziva, Roy R. Chaudhuri, Gemma C. Langridge, Daniel J. Turner, Derek J. Pickard, Duncan J. Maskell, Nicholas R. Thomson, Mark P. Stevens

Research output: Contribution to journalArticlepeer-review

Abstract

Massively parallel sequencing of transposon-flanking regions assigned the genotype and fitness score to 91% of Escherichia coli O157:H7 mutants previously screened in cattle by signature-tagged mutagenesis (STM). The method obviates the limitations of STM and markedly extended the functional annotation of the prototype E. coli O157:H7 genome without further animal use.
Original languageEnglish
Pages (from-to)1771-1776
Number of pages6
JournalJournal of Bacteriology
Volume193
Issue number7
DOIs
Publication statusPublished - Apr 2011

Keywords

  • Animals
  • Cattle
  • Cattle Diseases
  • Chromosomes, Bacterial
  • DNA Transposable Elements
  • DNA, Bacterial
  • Escherichia coli O157
  • Genetic Fitness
  • Genotype
  • Mutagenesis, Insertional
  • Plasmids

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