Retrospective detection by negative contrast electron microscopy of faecal viral particles in free-living wild red squirrels (Sciurus vulgaris) with suspected enteropathy in Great Britain

D.J. Everest; M.F. Stidworthy; Elspeth Milne; A.L. Meredith; J. Chantrey; C. Shuttleworth; T. Blackett; H. Butler; M. Wilkinson; A.W. Sainsbury

doi:10.1136/vr.c4111

Retrospective detection by negative contrast electron microscopy of faecal viral particles in free-living wild red squirrels (Sciurus vulgaris) with suspected enteropathy in Great Britain

D.J. Everest, M.F. Stidworthy, Elspeth Milne, A.L. Meredith, J. Chantrey, C. Shuttleworth, T. Blackett, H. Butler, M. Wilkinson, A.W. Sainsbury

Royal (Dick) School of Veterinary Studies

Research output: Contribution to journal › Article › peer-review

Abstract / Description of output

Transmission electron microscopy identified adenovirus particles in 10 of 70(14.3 per cent) samples of large intestinal content collected at postmortem examination from free-living wild red squirrels (Sciurus vulgoris) across Great Britain between 2000 and 2009. Examination was limited to cases in which an enteropathy was suspected on the basis of predetermined macroscopic criteria such as semi-solid or diarrhoeic faeces, suspected enteritis or the presence of intussusception. In most cases, meaningful histological examination of enteric tissue was not possible due to pronounced autolysis. Two (2.9 per cent) of the samples were negative for adenovirus but were found to contain rotavirus particles, a novel finding in this species.

Original language	Undefined/Unknown
Pages (from-to)	1007-1010
Number of pages	4
Journal	Veterinary Record
Volume	167
Issue number	26
DOIs	https://doi.org/10.1136/vr.c4111
Publication status	Published - Dec 2010

Access to Document

10.1136/vr.c4111

http://veterinaryrecord.bmj.com/content/167/26/1007.abstract

Cite this

Everest, D. J., Stidworthy, M. F., Milne, E., Meredith, A. L., Chantrey, J., Shuttleworth, C., Blackett, T., Butler, H., Wilkinson, M., & Sainsbury, A. W. (2010). Retrospective detection by negative contrast electron microscopy of faecal viral particles in free-living wild red squirrels (Sciurus vulgaris) with suspected enteropathy in Great Britain. Veterinary Record, 167(26), 1007-1010. https://doi.org/10.1136/vr.c4111

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title = "Retrospective detection by negative contrast electron microscopy of faecal viral particles in free-living wild red squirrels (Sciurus vulgaris) with suspected enteropathy in Great Britain",

abstract = "Transmission electron microscopy identified adenovirus particles in 10 of 70(14.3 per cent) samples of large intestinal content collected at postmortem examination from free-living wild red squirrels (Sciurus vulgoris) across Great Britain between 2000 and 2009. Examination was limited to cases in which an enteropathy was suspected on the basis of predetermined macroscopic criteria such as semi-solid or diarrhoeic faeces, suspected enteritis or the presence of intussusception. In most cases, meaningful histological examination of enteric tissue was not possible due to pronounced autolysis. Two (2.9 per cent) of the samples were negative for adenovirus but were found to contain rotavirus particles, a novel finding in this species.",

author = "D.J. Everest and M.F. Stidworthy and Elspeth Milne and A.L. Meredith and J. Chantrey and C. Shuttleworth and T. Blackett and H. Butler and M. Wilkinson and A.W. Sainsbury",

note = "ISI:000287630300016 Everest, D. J. Stidworthy, M. F. Milne, E. M. Meredith, A. L. Chantrey, J. Shuttleworth, C. Blackett, T. Butler, H. Wilkinson, M. Sainsbury, A. W.",

year = "2010",

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doi = "10.1136/vr.c4111",

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Everest, DJ, Stidworthy, MF, Milne, E, Meredith, AL, Chantrey, J, Shuttleworth, C, Blackett, T, Butler, H, Wilkinson, M & Sainsbury, AW 2010, 'Retrospective detection by negative contrast electron microscopy of faecal viral particles in free-living wild red squirrels (Sciurus vulgaris) with suspected enteropathy in Great Britain', Veterinary Record, vol. 167, no. 26, pp. 1007-1010. https://doi.org/10.1136/vr.c4111

Retrospective detection by negative contrast electron microscopy of faecal viral particles in free-living wild red squirrels (Sciurus vulgaris) with suspected enteropathy in Great Britain. / Everest, D.J.; Stidworthy, M.F.; Milne, Elspeth et al.
In: Veterinary Record, Vol. 167, No. 26, 12.2010, p. 1007-1010.

Research output: Contribution to journal › Article › peer-review

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T1 - Retrospective detection by negative contrast electron microscopy of faecal viral particles in free-living wild red squirrels (Sciurus vulgaris) with suspected enteropathy in Great Britain

AU - Everest, D.J.

AU - Stidworthy, M.F.

AU - Milne, Elspeth

AU - Meredith, A.L.

AU - Chantrey, J.

AU - Shuttleworth, C.

AU - Blackett, T.

AU - Butler, H.

AU - Wilkinson, M.

AU - Sainsbury, A.W.

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