Revealing the early stages of carbamazepine crystallization by cryoTEM and 3D electron diffraction

Edward T. Broadhurst; Hongyi Xu; Simon Parsons; Fabio Nudelman

doi:10.1107/S2052252521010101

Revealing the early stages of carbamazepine crystallization by cryoTEM and 3D electron diffraction

Edward T. Broadhurst, Hongyi Xu, Simon Parsons, Fabio Nudelman

Research output: Contribution to journal › Article › peer-review

Abstract / Description of output

Time-resolved carbamazepine crystallization from wet ethanol has been monitored using a combination of cryoTEM and 3D electron diffraction. Carbamazepine is shown to crystallize exclusively as a dihydrate after 180 s. When the timescale was reduced to 30 s, three further polymorphs could be identified. At 20 s, the development of early stage carbamazepine dihydrate was observed through phase separation. This work reveals two possible crystallization pathways present in this active pharmaceutical ingredient.

Original language	English
Journal	IUCrJ
Volume	8
Issue number	6
Early online date	14 Oct 2021
DOIs	https://doi.org/10.1107/S2052252521010101
Publication status	E-pub ahead of print - 14 Oct 2021

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10.1107/S2052252521010101

20211025_Nudelman_yc5034_VoRFinal published version, 1.16 MBLicence: Creative Commons: Attribution (CC-BY)

https://journals.iucr.org/m/issues/2021/06/00/yc5034/

Purchase of a high-resolution electron cryo-microscope
Böttcher, B., Earnshaw, B., Kohl, A., SPAGNOLO, L. & Walkinshaw, M.
UK-based charities
1/08/09 → 31/07/14
Project: Research

Cite this

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title = "Revealing the early stages of carbamazepine crystallization by cryoTEM and 3D electron diffraction",

abstract = "Time-resolved carbamazepine crystallization from wet ethanol has been monitored using a combination of cryoTEM and 3D electron diffraction. Carbamazepine is shown to crystallize exclusively as a dihydrate after 180 s. When the timescale was reduced to 30 s, three further polymorphs could be identified. At 20 s, the development of early stage carbamazepine dihydrate was observed through phase separation. This work reveals two possible crystallization pathways present in this active pharmaceutical ingredient.",

author = "Broadhurst, {Edward T.} and Hongyi Xu and Simon Parsons and Fabio Nudelman",

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doi = "10.1107/S2052252521010101",

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T1 - Revealing the early stages of carbamazepine crystallization by cryoTEM and 3D electron diffraction

AU - Broadhurst, Edward T.

AU - Xu, Hongyi

AU - Parsons, Simon

AU - Nudelman, Fabio

PY - 2021/10/14

Y1 - 2021/10/14

N2 - Time-resolved carbamazepine crystallization from wet ethanol has been monitored using a combination of cryoTEM and 3D electron diffraction. Carbamazepine is shown to crystallize exclusively as a dihydrate after 180 s. When the timescale was reduced to 30 s, three further polymorphs could be identified. At 20 s, the development of early stage carbamazepine dihydrate was observed through phase separation. This work reveals two possible crystallization pathways present in this active pharmaceutical ingredient.

AB - Time-resolved carbamazepine crystallization from wet ethanol has been monitored using a combination of cryoTEM and 3D electron diffraction. Carbamazepine is shown to crystallize exclusively as a dihydrate after 180 s. When the timescale was reduced to 30 s, three further polymorphs could be identified. At 20 s, the development of early stage carbamazepine dihydrate was observed through phase separation. This work reveals two possible crystallization pathways present in this active pharmaceutical ingredient.

U2 - 10.1107/S2052252521010101

DO - 10.1107/S2052252521010101

M3 - Article

SN - 2052-2525

VL - 8

JO - IUCrJ

JF - IUCrJ

IS - 6

ER -

Revealing the early stages of carbamazepine crystallization by cryoTEM and 3D electron diffraction

Abstract / Description of output

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Purchase of a high-resolution electron cryo-microscope

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