Reverse Phase Protein Arrays and Drug Discovery

Kenneth G. Macleod, Bryan Serrels, Neil O. Carragher

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Reverse Phase Protein Arrays (RPPA) represent a sensitive antibody-based proteomic approach, which enables simultaneous quantification of the abundance of multiple proteins and posttranslational modifications across multiple samples. Here, we provide protocols for RPPA performed on two distinct protein-binding substrates associated with two most commonly used RPPA platform technologies. We compare and contrast the respective advantages and limitations of each platform within the context of drug discovery applications.
Original languageEnglish
Title of host publicationProteomics for Drug Discovery
Pages153-169
DOIs
Publication statusPublished - 15 Aug 2017

Publication series

NameProteomics for Drug Discovery
Volume1647
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

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