Reverse Phase Protein Arrays elucidate mechanisms-of-action and phenotypic response in 2D and 3D models

Michael Pawlak, Neil O. Carragher

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

The development of new 2D and 3D phenotypic screening assays combined with high-throughput genomic and proteomic technologies are well placed to advance a new era of molecular pathway informed Phenotypic Drug Discovery. We describe the application of Reverse Phase Protein Array (RPPA) technology to elucidate the mechanism-of-action of small molecules at the post-translational pathway level. We propose that profiling of phenotypic hits and lead molecules in increasingly more complex 3D in vitro and ex vivo models at the post-translational pathway network level represents an effective strategy to both triage and progress the preclinical development of phenotypic screening hits.
Original languageEnglish
JournalDrug Discovery Today: Technologies
Early online date30 May 2017
DOIs
Publication statusE-pub ahead of print - 30 May 2017

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