Revision of the validity of CAMP tests for Listeria identification. Proposal of an alternative method for the determination of haemolytic activity by Listeria strains

Jose A Vazquez-Boland, L Dominguez, JF Fernandez, Elias Fernando Rodriguez-Ferri, Victor Briones, M. Blanco, G Suarez

Research output: Contribution to journalArticlepeer-review

Abstract

The validity of CAMP tests with Staphylococcus aureus and Rhodococcus (Corynebacterium) equi as defined for Listeria identification was revised. This characterization method appeared to be unreliable for two reasons: first, a positive CAMP test with R. equi is not specific for Listeria ivanovii as Listeria monocytogenes (and Listeria seeligeri) give also a clear positive reaction; second, doubtful reactions could be observed with S. aureus when assaying haemolytic and non-haemolytic Listeria strains (possibility of false negative and false positive results; subjectivity of the interpretation). The use of a Microplate technique previously described instead of CAMP tests is proposed for the reliable demonstration of the haemolytic character of Listeria in the routine identification of these organisms.
Original languageEnglish
Pages (from-to)201-6
Number of pages6
JournalActa Microbiologica Hungarica
Volume37
Issue number2
Publication statusPublished - 1990

Keywords

  • Bacterial Typing Techniques
  • Diagnostic Errors
  • Evaluation Studies as Topic
  • Food Microbiology
  • Hemolysis
  • Listeria/classification
  • Rhodococcus
  • Staphylococcus aureus

Fingerprint

Dive into the research topics of 'Revision of the validity of CAMP tests for Listeria identification. Proposal of an alternative method for the determination of haemolytic activity by Listeria strains'. Together they form a unique fingerprint.

Cite this