Revision of the validity of CAMP tests for Listeria identification. Proposal of an alternative method for the determination of haemolytic activity by Listeria strains

Jose A Vazquez-Boland; L Dominguez; JF Fernandez; Elias Fernando Rodriguez-Ferri; Victor Briones; M. Blanco; G Suarez

Revision of the validity of CAMP tests for Listeria identification. Proposal of an alternative method for the determination of haemolytic activity by Listeria strains

Jose A Vazquez-Boland, L Dominguez, JF Fernandez, Elias Fernando Rodriguez-Ferri, Victor Briones, M. Blanco, G Suarez

Deanery of Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

Abstract / Description of output

The validity of CAMP tests with Staphylococcus aureus and Rhodococcus (Corynebacterium) equi as defined for Listeria identification was revised. This characterization method appeared to be unreliable for two reasons: first, a positive CAMP test with R. equi is not specific for Listeria ivanovii as Listeria monocytogenes (and Listeria seeligeri) give also a clear positive reaction; second, doubtful reactions could be observed with S. aureus when assaying haemolytic and non-haemolytic Listeria strains (possibility of false negative and false positive results; subjectivity of the interpretation). The use of a Microplate technique previously described instead of CAMP tests is proposed for the reliable demonstration of the haemolytic character of Listeria in the routine identification of these organisms.

Original language	English
Pages (from-to)	201-6
Number of pages	6
Journal	Acta Microbiologica Hungarica
Volume	37
Issue number	2
Publication status	Published - 1990

Keywords / Materials (for Non-textual outputs)

Bacterial Typing Techniques
Diagnostic Errors
Evaluation Studies as Topic
Food Microbiology
Hemolysis
Listeria/classification
Rhodococcus
Staphylococcus aureus

Cite this

@article{3e1e8e03f8844a9db3213df05cafac92,

title = "Revision of the validity of CAMP tests for Listeria identification. Proposal of an alternative method for the determination of haemolytic activity by Listeria strains",

abstract = "The validity of CAMP tests with Staphylococcus aureus and Rhodococcus (Corynebacterium) equi as defined for Listeria identification was revised. This characterization method appeared to be unreliable for two reasons: first, a positive CAMP test with R. equi is not specific for Listeria ivanovii as Listeria monocytogenes (and Listeria seeligeri) give also a clear positive reaction; second, doubtful reactions could be observed with S. aureus when assaying haemolytic and non-haemolytic Listeria strains (possibility of false negative and false positive results; subjectivity of the interpretation). The use of a Microplate technique previously described instead of CAMP tests is proposed for the reliable demonstration of the haemolytic character of Listeria in the routine identification of these organisms.",

keywords = "Bacterial Typing Techniques, Diagnostic Errors, Evaluation Studies as Topic, Food Microbiology, Hemolysis, Listeria/classification, Rhodococcus, Staphylococcus aureus",

author = "Vazquez-Boland, {Jose A} and L Dominguez and JF Fernandez and Rodriguez-Ferri, {Elias Fernando} and Victor Briones and M. Blanco and G Suarez",

year = "1990",

language = "English",

volume = "37",

pages = "201--6",

journal = "Acta Microbiologica Hungarica",

issn = "0231-4622",

publisher = "Akademiai Kiado",

number = "2",

}

TY - JOUR

T1 - Revision of the validity of CAMP tests for Listeria identification. Proposal of an alternative method for the determination of haemolytic activity by Listeria strains

AU - Vazquez-Boland, Jose A

AU - Dominguez, L

AU - Fernandez, JF

AU - Rodriguez-Ferri, Elias Fernando

AU - Briones, Victor

AU - Blanco, M.

AU - Suarez, G

PY - 1990

Y1 - 1990

N2 - The validity of CAMP tests with Staphylococcus aureus and Rhodococcus (Corynebacterium) equi as defined for Listeria identification was revised. This characterization method appeared to be unreliable for two reasons: first, a positive CAMP test with R. equi is not specific for Listeria ivanovii as Listeria monocytogenes (and Listeria seeligeri) give also a clear positive reaction; second, doubtful reactions could be observed with S. aureus when assaying haemolytic and non-haemolytic Listeria strains (possibility of false negative and false positive results; subjectivity of the interpretation). The use of a Microplate technique previously described instead of CAMP tests is proposed for the reliable demonstration of the haemolytic character of Listeria in the routine identification of these organisms.

AB - The validity of CAMP tests with Staphylococcus aureus and Rhodococcus (Corynebacterium) equi as defined for Listeria identification was revised. This characterization method appeared to be unreliable for two reasons: first, a positive CAMP test with R. equi is not specific for Listeria ivanovii as Listeria monocytogenes (and Listeria seeligeri) give also a clear positive reaction; second, doubtful reactions could be observed with S. aureus when assaying haemolytic and non-haemolytic Listeria strains (possibility of false negative and false positive results; subjectivity of the interpretation). The use of a Microplate technique previously described instead of CAMP tests is proposed for the reliable demonstration of the haemolytic character of Listeria in the routine identification of these organisms.

KW - Bacterial Typing Techniques

KW - Diagnostic Errors

KW - Evaluation Studies as Topic

KW - Food Microbiology

KW - Hemolysis

KW - Listeria/classification

KW - Rhodococcus

KW - Staphylococcus aureus

M3 - Article

SN - 0231-4622

VL - 37

SP - 201

EP - 206

JO - Acta Microbiologica Hungarica

JF - Acta Microbiologica Hungarica

IS - 2

ER -

Revision of the validity of CAMP tests for Listeria identification. Proposal of an alternative method for the determination of haemolytic activity by Listeria strains

Abstract / Description of output

Keywords / Materials (for Non-textual outputs)

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