RNA-dependent RNA polymerase in nuclei of cells infected with influenza virus

N D Hastie, B W Mahy

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

Nuclei purified from chicken embryo fibroblast cells infected with influenza (fowl plague) virus contain an RNA-dependent RNA polymerase. The in vitro activity of this enzyme is insensitive to actinomycin D, and is completely destroyed by preincubation with ribonuclease. Enzyme induction is prevented if cells are treated with actinomycin D or cycloheximide at the time of infection. RNA-dependent RNA polymerase activity increases rapidly in cell nuclei from 1 h postinfection, reaches a maximum at 3 to 4 h, then declines; a similar RNA polymerase activity in the microsomal cell fraction increases from 2 h postinfection and reaches a maximum at 5 to 6 h. The characteristics of the nuclear and microsomal enzymes in vitro are similar with respect to pH and divalent cation requirements. The in vitro products of enzyme activity present in the nuclear and microsomal fractions of cells infected for 3 and 5 h were characterized by sucrose density gradient analysis, and annealing to virion RNA. The microsomal RNA polymerase product contained 67 and 93% RNA complementary to virion RNA at 3 and 5 h, respectively; for the nuclear RNA polymerase product these values were 40% in each case.

Original languageEnglish
Pages (from-to)951-61
Number of pages11
JournalJournal of Virology
Volume12
Issue number5
Publication statusPublished - Nov 1973

Keywords / Materials (for Non-textual outputs)

  • Animals
  • Cell Fractionation
  • Cell Nucleus
  • Centrifugation, Density Gradient
  • Chick Embryo
  • Cytochrome Reductases
  • Cytochrome c Group
  • DNA, Viral
  • DNA-Directed RNA Polymerases
  • Dactinomycin
  • Enzyme Induction
  • Fibroblasts
  • Hemagglutination Tests
  • Influenza A virus
  • Magnesium
  • Manganese
  • Microsomes
  • NAD
  • Nucleic Acid Hybridization
  • Orthomyxoviridae
  • Proteins
  • RNA
  • Sucrose
  • Time Factors
  • Tritium

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