RNA interference restricts Rift Valley fever virus in multiple insect systems

Isabelle Dietrich; Stephanie Jansen; Gamou Fall; Stephan Lorenzen; Martin Rudolf; Katrin Huber; Anna Heitmann; Sabine Schicht; El Hadji Ndiaye; Michael Watson; Ilaria Castelli; Benjamin Brennan; Richard M. Elliott; Mawlouth Diallo; Amadou A. Sall; Anna-Bella Failloux; Esther Schnettler; Alain Kohl; Stefanie C. Becker

doi:10.1128/mSphere.00090-17

RNA interference restricts Rift Valley fever virus in multiple insect systems

Isabelle Dietrich, Stephanie Jansen, Gamou Fall, Stephan Lorenzen, Martin Rudolf, Katrin Huber, Anna Heitmann, Sabine Schicht, El Hadji Ndiaye, Michael Watson, Ilaria Castelli, Benjamin Brennan, Richard M. Elliott, Mawlouth Diallo, Amadou A. Sall, Anna-Bella Failloux, Esther Schnettler, Alain Kohl, Stefanie C. Becker

Research output: Contribution to journal › Article › peer-review

Abstract

The emerging bunyavirus Rift Valley fever virus (RVFV) is transmitted to humans and livestock by a large number of mosquito species. RNA interference (RNAi) has been characterized as an important innate immune defense mechanism used by mosquitoes to limit replication of positive-sense RNA flavi- and togaviruses; however, little is known about its role against negative-strand RNA viruses such as RVFV. We show that virus-specific small RNAs are produced in infected mosquito cells, RVFV vector mosquitoes and those derived from the model insect Drosophila melanogaster. We also show that these have antiviral activity, since silencing of RNAi pathway effectors enhances viral replication. Moreover, our data suggest that RVFV does not encode for a suppressor of RNAi. These findings point towards a significant role of RNAi in the control of RVFV in mosquitoes.

Original language	English
Article number	e00090-17
Journal	mSphere
Volume	2
Issue number	3
Early online date	3 May 2017
DOIs	https://doi.org/10.1128/mSphere.00090-17
Publication status	E-pub ahead of print - 3 May 2017

Keywords

antiviral immunity
RNA interference
mosquito
Drosophila melanogaster
Rift Valley fever virus

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10.1128/mSphere.00090-17

RNA Interference Restricts Rift Valley Fever Virus in Multiple Insect Systems
© 2017 Dietrich et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.
Final published version, 1.6 MBLicence: Creative Commons: Attribution (CC-BY)

1 Active
2 Finished

ISP 2 2017/22 Control of Infectious Diseases
Gally, D.
BBSRC
1/04/17 → 31/03/22
Project: Research
Ark Genomics
Watson, M., Archibald, A. & Burt, D.
BBSRC
1/04/12 → 31/03/17
Project: Research
ISP4 Pathogenesis and resistance in viral diseases of livestock
Dutia, B., Archibald, A., Beard, P., Bishop, S., Bronsvoort, M., Burt, D., Collie, D., Digard, P., Freeman, T., Glass, E., Grey, F., Hocking, P., Houston, R., Hume, D., Kaiser, P., Nash, A., Sang, H., Sharp, C., Watson, M. & Whitelaw, B.
BBSRC
1/04/12 → 31/03/17
Project: Research

Cite this

Dietrich, I., Jansen, S., Fall, G., Lorenzen, S., Rudolf, M., Huber, K., Heitmann, A., Schicht, S., Ndiaye, E. H., Watson, M., Castelli, I., Brennan, B., Elliott, R. M., Diallo, M., Sall, A. A., Failloux, A-B., Schnettler, E., Kohl, A., & Becker, S. C. (2017). RNA interference restricts Rift Valley fever virus in multiple insect systems. mSphere, 2(3), [e00090-17]. https://doi.org/10.1128/mSphere.00090-17

Dietrich, Isabelle ; Jansen, Stephanie ; Fall, Gamou ; Lorenzen, Stephan ; Rudolf, Martin ; Huber, Katrin ; Heitmann, Anna ; Schicht, Sabine ; Ndiaye, El Hadji ; Watson, Michael ; Castelli, Ilaria ; Brennan, Benjamin ; Elliott, Richard M. ; Diallo, Mawlouth ; Sall, Amadou A. ; Failloux, Anna-Bella ; Schnettler, Esther ; Kohl, Alain ; Becker, Stefanie C. / RNA interference restricts Rift Valley fever virus in multiple insect systems. In: mSphere. 2017 ; Vol. 2, No. 3.

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title = "RNA interference restricts Rift Valley fever virus in multiple insect systems",

abstract = "The emerging bunyavirus Rift Valley fever virus (RVFV) is transmitted to humans and livestock by a large number of mosquito species. RNA interference (RNAi) has been characterized as an important innate immune defense mechanism used by mosquitoes to limit replication of positive-sense RNA flavi- and togaviruses; however, little is known about its role against negative-strand RNA viruses such as RVFV. We show that virus-specific small RNAs are produced in infected mosquito cells, RVFV vector mosquitoes and those derived from the model insect Drosophila melanogaster. We also show that these have antiviral activity, since silencing of RNAi pathway effectors enhances viral replication. Moreover, our data suggest that RVFV does not encode for a suppressor of RNAi. These findings point towards a significant role of RNAi in the control of RVFV in mosquitoes.",

keywords = "antiviral immunity, RNA interference, mosquito, Drosophila melanogaster, Rift Valley fever virus",

author = "Isabelle Dietrich and Stephanie Jansen and Gamou Fall and Stephan Lorenzen and Martin Rudolf and Katrin Huber and Anna Heitmann and Sabine Schicht and Ndiaye, {El Hadji} and Michael Watson and Ilaria Castelli and Benjamin Brennan and Elliott, {Richard M.} and Mawlouth Diallo and Sall, {Amadou A.} and Anna-Bella Failloux and Esther Schnettler and Alain Kohl and Becker, {Stefanie C.}",

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month = may,

day = "3",

doi = "10.1128/mSphere.00090-17",

language = "English",

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Dietrich, I, Jansen, S, Fall, G, Lorenzen, S, Rudolf, M, Huber, K, Heitmann, A, Schicht, S, Ndiaye, EH, Watson, M, Castelli, I, Brennan, B, Elliott, RM, Diallo, M, Sall, AA, Failloux, A-B, Schnettler, E, Kohl, A & Becker, SC 2017, 'RNA interference restricts Rift Valley fever virus in multiple insect systems', mSphere, vol. 2, no. 3, e00090-17. https://doi.org/10.1128/mSphere.00090-17

RNA interference restricts Rift Valley fever virus in multiple insect systems. / Dietrich, Isabelle; Jansen, Stephanie; Fall, Gamou; Lorenzen, Stephan; Rudolf, Martin; Huber, Katrin; Heitmann, Anna; Schicht, Sabine; Ndiaye, El Hadji; Watson, Michael; Castelli, Ilaria; Brennan, Benjamin; Elliott, Richard M.; Diallo, Mawlouth; Sall, Amadou A.; Failloux, Anna-Bella; Schnettler, Esther; Kohl, Alain; Becker, Stefanie C.

In: mSphere, Vol. 2, No. 3, e00090-17, 03.05.2017.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - RNA interference restricts Rift Valley fever virus in multiple insect systems

AU - Dietrich, Isabelle

AU - Jansen, Stephanie

AU - Fall, Gamou

AU - Lorenzen, Stephan

AU - Rudolf, Martin

AU - Huber, Katrin

AU - Heitmann, Anna

AU - Schicht, Sabine

AU - Ndiaye, El Hadji

AU - Watson, Michael

AU - Castelli, Ilaria

AU - Brennan, Benjamin

AU - Elliott, Richard M.

AU - Diallo, Mawlouth

AU - Sall, Amadou A.

AU - Failloux, Anna-Bella

AU - Schnettler, Esther

AU - Kohl, Alain

AU - Becker, Stefanie C.

PY - 2017/5/3

Y1 - 2017/5/3

N2 - The emerging bunyavirus Rift Valley fever virus (RVFV) is transmitted to humans and livestock by a large number of mosquito species. RNA interference (RNAi) has been characterized as an important innate immune defense mechanism used by mosquitoes to limit replication of positive-sense RNA flavi- and togaviruses; however, little is known about its role against negative-strand RNA viruses such as RVFV. We show that virus-specific small RNAs are produced in infected mosquito cells, RVFV vector mosquitoes and those derived from the model insect Drosophila melanogaster. We also show that these have antiviral activity, since silencing of RNAi pathway effectors enhances viral replication. Moreover, our data suggest that RVFV does not encode for a suppressor of RNAi. These findings point towards a significant role of RNAi in the control of RVFV in mosquitoes.

AB - The emerging bunyavirus Rift Valley fever virus (RVFV) is transmitted to humans and livestock by a large number of mosquito species. RNA interference (RNAi) has been characterized as an important innate immune defense mechanism used by mosquitoes to limit replication of positive-sense RNA flavi- and togaviruses; however, little is known about its role against negative-strand RNA viruses such as RVFV. We show that virus-specific small RNAs are produced in infected mosquito cells, RVFV vector mosquitoes and those derived from the model insect Drosophila melanogaster. We also show that these have antiviral activity, since silencing of RNAi pathway effectors enhances viral replication. Moreover, our data suggest that RVFV does not encode for a suppressor of RNAi. These findings point towards a significant role of RNAi in the control of RVFV in mosquitoes.

KW - antiviral immunity

KW - RNA interference

KW - mosquito

KW - Drosophila melanogaster

KW - Rift Valley fever virus

U2 - 10.1128/mSphere.00090-17

DO - 10.1128/mSphere.00090-17

M3 - Article

VL - 2

JO - mSphere

JF - mSphere

SN - 2379-5042

IS - 3

M1 - e00090-17

ER -

RNA interference restricts Rift Valley fever virus in multiple insect systems

Abstract

Keywords

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Projects

ISP 2 2017/22 Control of Infectious Diseases

Ark Genomics

ISP4 Pathogenesis and resistance in viral diseases of livestock

Cite this