Projects per year
Abstract / Description of output
RNA localisation is a key mechanism for targeting proteins to particular sub-cellular domains. Sequences necessary and sufficient for localisation have been identified but little is known about factors that affect its kinetics. Transcripts of gurken and the I factor, a non-LTR retrotransposon, co-localise at the nucleus in the dorso-antero corner of the Drosophila oocyte directed by localisation signals, the GLS and ILS. I factor RNA localises faster than gurken after injection into oocytes due to a difference in the intrinsic localisation ability of the GLS and ILS. The kinetics of localisation of RNA containing the ILS are enhanced by the presence of a stem loop, the A loop. This acts as an RNA:RNA interaction element in vivo and in vitro, and stimulates localisation of RNA containing other localisation signals. RNA:RNA interaction may be a general mechanism for modulating RNA localisation and could allow an mRNA that lacks a localisation signal to hitchhike on another RNA that has one.
Original language | English |
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Pages (from-to) | 729-737 |
Number of pages | 9 |
Journal | RNA |
Volume | 18 |
Issue number | 4 |
DOIs | |
Publication status | Published - 2012 |
Keywords / Materials (for Non-textual outputs)
- Drosophila oocyte
- I factor
- RNA localization
- RNA:RNA interaction
- gurken
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- 1 Finished
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RNA Localisation and transposition of a non-LTR retrotransposon
Finnegan, D.
1/10/07 → 30/11/10
Project: Research