Abstract
Urokinase-type plasminogen activator (uPA) mRNA is induced in macrophages by the lineage specific growth factor CSF-1. Upon removal of CSF-1 from bone marrow-derived macrophages (BMM), uPA mRNA decayed with a half-life of 2 h. If RNA synthesis inhibitors actinomycin D, 5,6-dichloro-1-beta-ribofuranosyl benzimidazole (DRB) or alpha-amanitin were added at the time as CSF-1 removal, the uPA message was stabilised. This was not a general effect on CSF-1 responsive mRNAs, as c-myc mRNA decayed with normal kinetics in the presence of inhibitors. The requirement for ongoing RNA synthesis for the degradation of uPA mRNA in BMM suggests that a component of the degradative pathway may be induced following removal of CSF-1.
Original language | English |
---|---|
Pages (from-to) | 311-3 |
Number of pages | 3 |
Journal | FEBS Letters |
Volume | 356 |
Issue number | 2-3 |
Publication status | Published - 19 Dec 1994 |
Keywords / Materials (for Non-textual outputs)
- Amanitins
- Animals
- Base Sequence
- Bone Marrow
- Cell Differentiation
- Dactinomycin
- Dichlororibofuranosylbenzimidazole
- Enzyme Induction
- Humans
- Kinetics
- Macrophage Colony-Stimulating Factor
- Macrophages
- Mice
- Mice, Inbred BALB C
- Molecular Sequence Data
- Oligodeoxyribonucleotides
- RNA
- RNA, Messenger
- Recombinant Proteins
- Urokinase-Type Plasminogen Activator