RNA:DNA hybrids are a novel molecular pattern sensed by TLR9

Rachel E Rigby, Lauren M Webb, Karen J Mackenzie, Yue Li, Andrea Leitch, Martin A M Reijns, Rachel J Lundie, Ailsa Revuelta, Donald J Davidson, Sandra Diebold, Yorgo Modis, Andrew S MacDonald, Andrew P Jackson

Research output: Contribution to journalArticlepeer-review

Abstract

The sensing of nucleic acids by receptors of the innate immune system is a key component of antimicrobial immunity. RNA:DNA hybrids, as essential intracellular replication intermediates generated during infection, could therefore represent a class of previously uncharacterised pathogen‐associated molecular patterns sensed by pattern recognition receptors. Here we establish that RNA:DNA hybrids containing viral‐derived sequences efficiently induce pro‐inflammatory cytokine and antiviral type I interferon production in dendritic cells. We demonstrate that MyD88‐dependent signalling is essential for this cytokine response and identify TLR9 as a specific sensor of RNA:DNA hybrids. Hybrids therefore represent a novel molecular pattern sensed by the innate immune system and so could play an important role in host response to viruses and the pathogenesis of autoimmune disease.
Original languageEnglish
Pages (from-to)542-558
Number of pages17
JournalEMBO Journal
Volume33
Issue number6
Early online date21 Feb 2014
DOIs
Publication statusPublished - 18 Mar 2014

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