Projects per year
Abstract
immunohistochemistry, in situ hybridization, radiography, and microcomputed tomography. Phospho1 gene and PHOSPHO1 protein were expressed by active alveolar bone osteoblasts and cementoblasts during cellular cementum formation. In Phospho1‐/‐ mice, acellular cementum formation and
mineralization were unaffected while cellular cementum deposition increased, though displayed delayed mineralization and cementoid. Phospho1‐/‐ mice featured disturbances in alveolar bone mineralization, shown by accumulation of unmineralized osteiod matrix and interglobular patterns of protein deposition. Parallel to other skeletal sites, deposition of mineral‐regulating protein osteopontin (OPN) was increased in alveolar bone in Phospho1‐/‐ mice. In contrast to the skeleton, genetic ablation of Spp1, the gene encoding OPN, did not ameliorate dentoalveolar defects in Phospho1‐/‐ mice. Despite alveolar bone mineralization defects, periodontal attachment and function appeared undisturbed in Phospho1‐/‐ mice, with normal PDL architecture and no evidence of bone loss over time. This study highlights the role of PHOSPHO1 in mineralization of alveolar bone and cellular cementum, further revealing that acellular cementum formation is not substantially regulated by PHOSPHO1 and likely does not rely on matrix MV‐mediated initiation of mineralization.
Original language | English |
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Pages (from-to) | 742-751 |
Journal | Journal of Dental Research |
Volume | 95 |
Issue number | 7 |
Early online date | 25 Mar 2016 |
DOIs | |
Publication status | Published - Jul 2016 |
Keywords / Materials (for Non-textual outputs)
- PHospho1
- teeth
- extracellular matrix
- periodontal ligament
- dentin
- cementum
- bone
- physiologic calcification
Fingerprint
Dive into the research topics of 'Role of PHOSPHO1 in periodontal development and function'. Together they form a unique fingerprint.Projects
- 1 Finished
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Control of development and reproductive traits
Burdon, T. (Principal Investigator), Argyle, D. (Co-investigator), Ashworth, C. (Co-investigator), Beard, P. (Co-investigator), Brunton, P. (Co-investigator), Burt, D. (Co-investigator), Clinton, M. (Co-investigator), Dunn, I. (Co-investigator), Farquharson, C. (Co-investigator), Headon, D. (Co-investigator), Hocking, P. (Co-investigator), Hohenstein, P. (Co-investigator), Hume, D. (Co-investigator), Jackson, I. (Co-investigator), McColl, B. (Co-investigator), McGrew, M. (Co-investigator), McLachlan, G. (Co-investigator), Sang, H. (Co-investigator), Summers, K. (Co-investigator) & Whitelaw, B. (Co-investigator)
1/04/12 → 31/03/17
Project: Research
Research output
- 1 Article
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PLA2 and ENPP6 may act in concert to generate phosphocholine from the matrix vesicle membrane during skeletal mineralization
Stewart, A., Leong, D. & Farquharson, C., Jan 2018, In: The FASEB Journal. 32, 1, p. 20-25Research output: Contribution to journal › Article › peer-review