Role of PSIP1/LEDGF/p75 in lentiviral infectivity and integration targeting

Heather M Marshall; Keshet Ronen; Charles Berry; Manuel Llano; Heidi Sutherland; Dyana Saenz; Wendy Bickmore; Eric Poeschla; Frederic D Bushman

doi:10.1371/journal.pone.0001340

Role of PSIP1/LEDGF/p75 in lentiviral infectivity and integration targeting

Heather M Marshall, Keshet Ronen, Charles Berry, Manuel Llano, Heidi Sutherland, Dyana Saenz, Wendy Bickmore, Eric Poeschla, Frederic D Bushman

Research output: Contribution to journal › Article › peer-review

Abstract

To replicate, lentiviruses such as HIV must integrate DNA copies of their RNA genomes into host cell chromosomes. Lentiviral integration is favored in active transcription units, which allows efficient viral gene expression after integration, but the mechanisms directing integration targeting are incompletely understood. A cellular protein, PSIP1/LEDGF/p75, binds tightly to the lentiviral-encoded integrase protein (IN), and has been reported to be important for HIV infectivity and integration targeting.

Original language	English
Pages (from-to)	e1340
Journal	PLoS ONE
Volume	2
Issue number	12
DOIs	https://doi.org/10.1371/journal.pone.0001340
Publication status	Published - 2007

Keywords / Materials (for Non-textual outputs)

Adaptor Proteins, Signal Transducing
Animals
Cell Line
Consensus Sequence
HIV
Humans
Infectious Anemia Virus, Equine
Mice
Transcription Factors
Virulence
Virus Integration

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10.1371/journal.pone.0001340

Role of PSIP1 LEDGF p75 in lentiviral infectivity and integration targeting
Copyright: © 2007 Marshall et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Final published version, 417 KBLicence: Creative Commons: Attribution (CC-BY)

http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0001340

Cite this

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title = "Role of PSIP1/LEDGF/p75 in lentiviral infectivity and integration targeting",

abstract = "To replicate, lentiviruses such as HIV must integrate DNA copies of their RNA genomes into host cell chromosomes. Lentiviral integration is favored in active transcription units, which allows efficient viral gene expression after integration, but the mechanisms directing integration targeting are incompletely understood. A cellular protein, PSIP1/LEDGF/p75, binds tightly to the lentiviral-encoded integrase protein (IN), and has been reported to be important for HIV infectivity and integration targeting.",

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AU - Marshall, Heather M

AU - Ronen, Keshet

AU - Berry, Charles

AU - Llano, Manuel

AU - Sutherland, Heidi

AU - Saenz, Dyana

AU - Bickmore, Wendy

AU - Poeschla, Eric

AU - Bushman, Frederic D

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AB - To replicate, lentiviruses such as HIV must integrate DNA copies of their RNA genomes into host cell chromosomes. Lentiviral integration is favored in active transcription units, which allows efficient viral gene expression after integration, but the mechanisms directing integration targeting are incompletely understood. A cellular protein, PSIP1/LEDGF/p75, binds tightly to the lentiviral-encoded integrase protein (IN), and has been reported to be important for HIV infectivity and integration targeting.

KW - Adaptor Proteins, Signal Transducing

KW - Animals

KW - Cell Line

KW - Consensus Sequence

KW - HIV

KW - Humans

KW - Infectious Anemia Virus, Equine

KW - Mice

KW - Transcription Factors

KW - Virulence

KW - Virus Integration

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JO - PLoS ONE

JF - PLoS ONE

IS - 12

ER -

Role of PSIP1/LEDGF/p75 in lentiviral infectivity and integration targeting

Abstract

Keywords / Materials (for Non-textual outputs)

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