Role of the Talaromyces marneffei (Penicillium marneffei) sakA gene in nitrosative stress response, conidiation and red pigment production

Panjaphorn Nimmanee, Emily W T Tam, Patrick C Y Woo, Pramote Vanittanakom, Nongnuch Vanittanakom

Research output: Contribution to journalArticlepeer-review

Abstract

Stress-activated MAPK (SAPK) pathways are systems used to regulate the stress adaptation of most fungi. It has been shown that in Talaromyces marneffei (Penicillium marneffei), a pathogenic dimorphic fungus, the sakA gene is involved, not only in tolerance against oxidative and heat stresses, but also in playing a role in asexual development, yeast cell generation in vitro and survival inside macrophage cell lines. In this study, the role of the T. marneffei sakA gene on the nitrosative stress response, and the regulation of gene expression were investigated. The susceptibility of the sakA mutant to NaNO2 was investigated using drop dilution assay and the expression of genes of interest were determined by RT-PCR, comparing them to the wild type and complemented strains. The results demonstrated that the T. marneffei sakA gene played a role in the stress response to NaNO2, the expression of genes functioning in conidial development (brlA, abaA and wetA) and red pigment biosynthesis (pks3, rp1, rp2 and rp3). These findings suggest that T. marneffei sakA is broadly involved in a wide variety of cell activities, including stress response, cell morphogenesis, asexual development and pigmentation.
Original languageEnglish
Article numberfnw292
JournalFEMS Microbiology Letters
Early online date22 Dec 2016
DOIs
Publication statusE-pub ahead of print - 22 Dec 2016
Externally publishedYes

Keywords

  • sakA
  • stress response
  • nitrosative stress
  • red pigment biosynthesis
  • asexual development

Fingerprint Dive into the research topics of 'Role of the Talaromyces marneffei (Penicillium marneffei) sakA gene in nitrosative stress response, conidiation and red pigment production'. Together they form a unique fingerprint.

Cite this