Routes to induced pluripotent stem cells

Tyson Ruetz, Keisuke Kaji

Research output: Contribution to journalLiterature reviewpeer-review

Abstract / Description of output

The generation of induced pluripotent stem cells (iPSCs) with Oct4, Sox2, Klf4, c-Myc has been described as 'direct' reprogramming in contrast to reprogramming via nuclear transfer. Interestingly, recent studies have suggested that the conversion process itself includes transient up-regulation and down-regulation of hundreds of genes, making unique intermediate populations. In a sense, the process of 4 factor reprogramming is indirect. Like in vitro differentiation, iPSC generation efficiency and kinetics largely depend on the external environment, as well as the amount and stoichiometry of exogenously expressed reprogramming factors. However, accumulating evidence indicates that when reprogramming succeeds, the process is not random but progresses in an ordered, step-wise manner. In this review, we summarize current knowledge detailing how somatic cells reach a pluripotent state.

Original languageEnglish
Pages (from-to)38-42
Number of pages5
JournalCurrent Opinion in Genetics and Development
Volume28
DOIs
Publication statusPublished - Oct 2014

Keywords / Materials (for Non-textual outputs)

  • Animals
  • Cell Lineage
  • Cellular Reprogramming
  • Humans
  • Pluripotent Stem Cells

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