Run-to-tumble variability controls the surface residence times of E. coli bacteria

Gaspard Junot; Thierry Darnige; Anke Lindner; Vincent A. Martinez; Jochen Arlt; Angela Dawson; Wilson C. K. Poon; Harold Auradou; Eric Clément

doi:10.1103/PhysRevLett.128.248101

Run-to-tumble variability controls the surface residence times of E. coli bacteria

Gaspard Junot, Thierry Darnige, Anke Lindner, Vincent A. Martinez, Jochen Arlt, Angela Dawson, Wilson C. K. Poon, Harold Auradou, Eric Clément

School of Physics and Astronomy

Research output: Contribution to journal › Article › peer-review

Abstract

Motile bacteria are known to accumulate at surfaces, eventually leading to changes in bacterial motility and bio-film formation. We use a novel two-colour, three-dimensional Lagrangian tracking technique, to follow simultaneously the body and the agella of a wild-type Escherichia coli. We observe long surface residence times and surface escape corresponding mostly to immediately antecedent tumbling. A motility model accounting for a large behavioural variability in run-time duration, reproduces all experimental findings and gives new insights into surface trapping efficiency.

Original language	English
Article number	248101
Pages (from-to)	1-6
Number of pages	6
Journal	Physical Review Letters
Volume	128
Issue number	24
Early online date	14 Jun 2022
DOIs	https://doi.org/10.1103/PhysRevLett.128.248101
Publication status	Published - 17 Jun 2022

Keywords / Materials (for Non-textual outputs)

cond-mat.soft
physics.bio-ph

Access to Document

10.1103/PhysRevLett.128.248101

2107.11123v1Submitted manuscript, 5.73 MB
bacteria_surf_v24Accepted author manuscript, 5.53 MB

Cite this

@article{4c56108eec8442738344a759006714e2,

title = "Run-to-tumble variability controls the surface residence times of E. coli bacteria",

abstract = "Motile bacteria are known to accumulate at surfaces, eventually leading to changes in bacterial motility and bio-film formation. We use a novel two-colour, three-dimensional Lagrangian tracking technique, to follow simultaneously the body and the agella of a wild-type Escherichia coli. We observe long surface residence times and surface escape corresponding mostly to immediately antecedent tumbling. A motility model accounting for a large behavioural variability in run-time duration, reproduces all experimental findings and gives new insights into surface trapping efficiency.",

keywords = "cond-mat.soft, physics.bio-ph",

author = "Gaspard Junot and Thierry Darnige and Anke Lindner and Martinez, \{Vincent A.\} and Jochen Arlt and Angela Dawson and Poon, \{Wilson C. K.\} and Harold Auradou and Eric Cl{\'e}ment",

note = "Funding Information: This work was supported by Grant No. ANR-15-CE30-0013, “BacFlow” CNRS/Royal Society Grants No. PHC-1576 and No. IE160675, and the Institut Pierre-Gilles de Gennes (Investissements d{\textquoteright}avenir ANR-10-EQPX-34). A. L. and G. J. acknowledge support from the ERC Consolidator Grant PaDyFlow under Grant Agreement No. 682367. E. C. is supported by Institut Universitaire de France. V. A. M., J. A., A. D., and W. C. K. P. were funded by ERC (AdG 340877 PHYSAPS). Publisher Copyright: {\textcopyright} 2022 American Physical Society.",

year = "2022",

month = jun,

day = "17",

doi = "10.1103/PhysRevLett.128.248101",

language = "English",

volume = "128",

pages = "1--6",

journal = "Physical Review Letters",

issn = "0031-9007",

publisher = "American Physical Society",

number = "24",

}

TY - JOUR

T1 - Run-to-tumble variability controls the surface residence times of E. coli bacteria

AU - Junot, Gaspard

AU - Darnige, Thierry

AU - Lindner, Anke

AU - Martinez, Vincent A.

AU - Arlt, Jochen

AU - Dawson, Angela

AU - Poon, Wilson C. K.

AU - Auradou, Harold

AU - Clément, Eric

N1 - Funding Information: This work was supported by Grant No. ANR-15-CE30-0013, “BacFlow” CNRS/Royal Society Grants No. PHC-1576 and No. IE160675, and the Institut Pierre-Gilles de Gennes (Investissements d’avenir ANR-10-EQPX-34). A. L. and G. J. acknowledge support from the ERC Consolidator Grant PaDyFlow under Grant Agreement No. 682367. E. C. is supported by Institut Universitaire de France. V. A. M., J. A., A. D., and W. C. K. P. were funded by ERC (AdG 340877 PHYSAPS). Publisher Copyright: © 2022 American Physical Society.

PY - 2022/6/17

Y1 - 2022/6/17

N2 - Motile bacteria are known to accumulate at surfaces, eventually leading to changes in bacterial motility and bio-film formation. We use a novel two-colour, three-dimensional Lagrangian tracking technique, to follow simultaneously the body and the agella of a wild-type Escherichia coli. We observe long surface residence times and surface escape corresponding mostly to immediately antecedent tumbling. A motility model accounting for a large behavioural variability in run-time duration, reproduces all experimental findings and gives new insights into surface trapping efficiency.

AB - Motile bacteria are known to accumulate at surfaces, eventually leading to changes in bacterial motility and bio-film formation. We use a novel two-colour, three-dimensional Lagrangian tracking technique, to follow simultaneously the body and the agella of a wild-type Escherichia coli. We observe long surface residence times and surface escape corresponding mostly to immediately antecedent tumbling. A motility model accounting for a large behavioural variability in run-time duration, reproduces all experimental findings and gives new insights into surface trapping efficiency.

KW - cond-mat.soft

KW - physics.bio-ph

U2 - 10.1103/PhysRevLett.128.248101

DO - 10.1103/PhysRevLett.128.248101

M3 - Article

SN - 0031-9007

VL - 128

SP - 1

EP - 6

JO - Physical Review Letters

JF - Physical Review Letters

IS - 24

M1 - 248101

ER -

Run-to-tumble variability controls the surface residence times of E. coli bacteria

Abstract

Keywords / Materials (for Non-textual outputs)

Access to Document

Other files and links

Fingerprint

PHYSAPS: The Physics of Active Particle Suspensions

Cite this

Run-to-tumble variability controls the surface residence times of E. coli bacteria

Abstract

Keywords / Materials (for Non-textual outputs)

Access to Document

Other files and links

Fingerprint

Projects

PHYSAPS: The Physics of Active Particle Suspensions

Cite this