Projects per year
Abstract
Nitrogen assimilation plays a vital role in plant metabolism. Assimilation of nitrate, the primary source of nitrogen in soil, is linked to the generation of the redox signal nitric oxide (NO). An important mechanism by which NO regulates plant development and stress responses is through S-nitrosylation, that is, covalent attachment of NO to cysteine residues to form S-nitrosothiols (SNO). Despite the importance of nitrogen assimilation and NO signalling, it remains largely unknown how these pathways are interconnected. Here we show that SNO signalling suppresses both nitrate uptake and reduction by transporters and reductases, respectively, to fine tune nitrate homeostasis. Moreover, NO derived from nitrate assimilation suppresses the redox enzyme S-nitrosoglutathione Reductase 1 (GSNOR1) by S-nitrosylation, preventing scavenging of S-nitrosoglutathione, a major cellular bio-reservoir of NO. Hence, our data demonstrates that (S)NO controls its own generation and scavenging by modulating nitrate assimilation and GSNOR1 activity.
Original language | English |
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Article number | 5401 |
Journal | Nature Communications |
Volume | 5 |
DOIs | |
Publication status | Published - 11 Nov 2014 |
Fingerprint
Dive into the research topics of 'S-nitrosothiols regulate nitric oxide production and storage in plants through the nitrogen assimilation pathway'. Together they form a unique fingerprint.Projects
- 3 Finished
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SNO-CHIP & RED-CHIP: Novel methodologies for visualizing redox-mediated chromatin remodelling
1/05/12 → 30/04/14
Project: Research
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Redox Defence: towards a sustainable protection strategy against plant disease
1/05/11 → 30/04/12
Project: Research
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Research output
- 1 Chapter
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Control of nitrogen assimilation in plants through S-nitrosothiols
Frungillo, L., Spoel, S. H. & Salgado, I., 10 Feb 2016, Nitric Oxide and Signalling in Plants. Wendehenne, D. (ed.). Elsevier, p. 55-78 24 p. (Advances in Botanical Research; vol. 77).Research output: Chapter in Book/Report/Conference proceeding › Chapter
Press/Media
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Dr Steven Spoel leads study into absorption of nitrogen in crop plants
12/11/14
5 items of Media coverage
Press/Media: Research
Profiles
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Steven Spoel
- School of Biological Sciences - Personal Chair of Cell Signalling and Proteostasis
Person: Academic: Research Active