S-nitrosothiols regulate nitric oxide production and storage in plants through the nitrogen assimilation pathway

Lucas Frungillo, Michael Skelly, Gary J Loake, Steven H Spoel*, Ione Salgado

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

Nitrogen assimilation plays a vital role in plant metabolism. Assimilation of nitrate, the primary source of nitrogen in soil, is linked to the generation of the redox signal nitric oxide (NO). An important mechanism by which NO regulates plant development and stress responses is through S-nitrosylation, that is, covalent attachment of NO to cysteine residues to form S-nitrosothiols (SNO). Despite the importance of nitrogen assimilation and NO signalling, it remains largely unknown how these pathways are interconnected. Here we show that SNO signalling suppresses both nitrate uptake and reduction by transporters and reductases, respectively, to fine tune nitrate homeostasis. Moreover, NO derived from nitrate assimilation suppresses the redox enzyme S-nitrosoglutathione Reductase 1 (GSNOR1) by S-nitrosylation, preventing scavenging of S-nitrosoglutathione, a major cellular bio-reservoir of NO. Hence, our data demonstrates that (S)NO controls its own generation and scavenging by modulating nitrate assimilation and GSNOR1 activity.

Original languageEnglish
Article number5401
JournalNature Communications
Volume5
DOIs
Publication statusPublished - 11 Nov 2014

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