TY - JOUR
T1 - Salmonella Enteritidis-induced alteration of inflammatory CXCL chemokine messenger-RNA expression and histologic changes in the ceca of infected chicks
AU - Cheeseman, J.H.
AU - Levy, N.A.
AU - Kaiser, P.
AU - Lillehoj, H.S.
AU - Lamont, S.J.
N1 - 18646451
Cheeseman, Jennifer H Levy, Nyssa A Kaiser, Pete Lillehoj, Hyun S Lamont, Susan J Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. United States Avian diseases Avian Dis. 2008 Jun;52(2):229-34.
PY - 2008
Y1 - 2008
N2 - To understand better the events in early avian host immune responses to Salmonella Enteritidis (SE), we examined messenger-RNA (mRNA) expression for eight genes: CXCLi1[K60], CXCLi2 [IL-8/CAF], interferon (IFN)-gamma, interleukin (IL)-1beta, IL-6, IL-12alpha, IL-12beta, and gallinacin (Gal)-2 in the ceca of young chicks 1 wk postinoculation with SE. Cecum tissue sections were stained and evaluated for the presence of macrophages, lymphocytes, heterophils, and apoptotic cells following SE infection. With the use of quantitative reverse transcriptase-polymerase chain reaction (RT-PCR), SE infection was associated with a significant (P <0.01) upregulation of cecal CXCLi1 and CXCLi2 mRNA expression. Infection with SE was also associated (P <0.05) with increased staining for macrophages and decreased apoptosis (single-stranded DNA [ssDNA]) in cecal tissue sections when these sections were compared with those of uninfected animals. Changes in chemokine expression and cell population dynamics are a direct result of SE infection, as uninfected animals do not show these alterations. Thus, these SE-induced changes reflect the host immune response to SE in young chickens.
AB - To understand better the events in early avian host immune responses to Salmonella Enteritidis (SE), we examined messenger-RNA (mRNA) expression for eight genes: CXCLi1[K60], CXCLi2 [IL-8/CAF], interferon (IFN)-gamma, interleukin (IL)-1beta, IL-6, IL-12alpha, IL-12beta, and gallinacin (Gal)-2 in the ceca of young chicks 1 wk postinoculation with SE. Cecum tissue sections were stained and evaluated for the presence of macrophages, lymphocytes, heterophils, and apoptotic cells following SE infection. With the use of quantitative reverse transcriptase-polymerase chain reaction (RT-PCR), SE infection was associated with a significant (P <0.01) upregulation of cecal CXCLi1 and CXCLi2 mRNA expression. Infection with SE was also associated (P <0.05) with increased staining for macrophages and decreased apoptosis (single-stranded DNA [ssDNA]) in cecal tissue sections when these sections were compared with those of uninfected animals. Changes in chemokine expression and cell population dynamics are a direct result of SE infection, as uninfected animals do not show these alterations. Thus, these SE-induced changes reflect the host immune response to SE in young chickens.
M3 - Article
SN - 0005-2086
VL - 52
SP - 229
EP - 234
JO - Avian Diseases
JF - Avian Diseases
IS - 2
ER -