Salmonella Typhi sense host neuroendocrine stress hormones and release the toxin haemolysin E

Michail H Karavolos; David M Bulmer; Hannah Spencer; Giordano Rampioni; Ira Schmalen; Stephen Baker; Derek Pickard; Joe Gray; Maria Fookes; Klaus Winzer; Alasdair Ivens; Gordon Dougan; Paul Williams; C M Anjam Khan

doi:10.1038/embor.2011.4

Salmonella Typhi sense host neuroendocrine stress hormones and release the toxin haemolysin E

Michail H Karavolos, David M Bulmer, Hannah Spencer, Giordano Rampioni, Ira Schmalen, Stephen Baker, Derek Pickard, Joe Gray, Maria Fookes, Klaus Winzer, Alasdair Ivens, Gordon Dougan, Paul Williams, C M Anjam Khan

Research output: Contribution to journal › Article › peer-review

Abstract

Salmonella enterica serovar Typhi (S. typhi) causes typhoid fever. We show that exposure of S. typhi to neuroendocrine stress hormones results in haemolysis, which is associated with the release of haemolysin E in membrane vesicles. This effect is attributed to increased expression of the small RNA micA and RNA chaperone Hfq, with concomitant downregulation of outer membrane protein A. Deletion of micA or the two-component signal-transduction system, CpxAR, abolishes the phenotype. The hormone response is inhibited by the β-blocker propranolol. We provide mechanistic insights into the basis of neuroendocrine hormone-mediated haemolysis by S. typhi, increasing our understanding of inter-kingdom signalling.

Original language	English
Pages (from-to)	252-8
Number of pages	7
Journal	EMBO Reports
Volume	12
Issue number	3
DOIs	https://doi.org/10.1038/embor.2011.4
Publication status	Published - Mar 2011

Keywords / Materials (for Non-textual outputs)

Gene Expression Regulation, Bacterial
Salmonella typhi
Hormones
Bacterial Proteins
Down-Regulation
Propranolol
Norepinephrine
Hemolysis
Protein Kinases
Hemolysin Proteins
Bacterial Outer Membrane Proteins
Epinephrine
Signal Transduction
Stress, Physiological

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10.1038/embor.2011.4

http://www.nature.com/embor/journal/v12/n3/full/embor20114a.html

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title = "Salmonella Typhi sense host neuroendocrine stress hormones and release the toxin haemolysin E",

abstract = "Salmonella enterica serovar Typhi (S. typhi) causes typhoid fever. We show that exposure of S. typhi to neuroendocrine stress hormones results in haemolysis, which is associated with the release of haemolysin E in membrane vesicles. This effect is attributed to increased expression of the small RNA micA and RNA chaperone Hfq, with concomitant downregulation of outer membrane protein A. Deletion of micA or the two-component signal-transduction system, CpxAR, abolishes the phenotype. The hormone response is inhibited by the β-blocker propranolol. We provide mechanistic insights into the basis of neuroendocrine hormone-mediated haemolysis by S. typhi, increasing our understanding of inter-kingdom signalling.",

keywords = "Gene Expression Regulation, Bacterial, Salmonella typhi, Hormones, Bacterial Proteins, Down-Regulation, Propranolol, Norepinephrine, Hemolysis, Protein Kinases, Hemolysin Proteins, Bacterial Outer Membrane Proteins, Epinephrine, Signal Transduction, Stress, Physiological",

author = "Karavolos, {Michail H} and Bulmer, {David M} and Hannah Spencer and Giordano Rampioni and Ira Schmalen and Stephen Baker and Derek Pickard and Joe Gray and Maria Fookes and Klaus Winzer and Alasdair Ivens and Gordon Dougan and Paul Williams and Khan, {C M Anjam}",

year = "2011",

month = mar,

doi = "10.1038/embor.2011.4",

language = "English",

volume = "12",

pages = "252--8",

journal = "EMBO Reports",

issn = "1469-221X",

publisher = "Wiley",

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TY - JOUR

T1 - Salmonella Typhi sense host neuroendocrine stress hormones and release the toxin haemolysin E

AU - Karavolos, Michail H

AU - Bulmer, David M

AU - Spencer, Hannah

AU - Rampioni, Giordano

AU - Schmalen, Ira

AU - Baker, Stephen

AU - Pickard, Derek

AU - Gray, Joe

AU - Fookes, Maria

AU - Winzer, Klaus

AU - Ivens, Alasdair

AU - Dougan, Gordon

AU - Williams, Paul

AU - Khan, C M Anjam

PY - 2011/3

Y1 - 2011/3

N2 - Salmonella enterica serovar Typhi (S. typhi) causes typhoid fever. We show that exposure of S. typhi to neuroendocrine stress hormones results in haemolysis, which is associated with the release of haemolysin E in membrane vesicles. This effect is attributed to increased expression of the small RNA micA and RNA chaperone Hfq, with concomitant downregulation of outer membrane protein A. Deletion of micA or the two-component signal-transduction system, CpxAR, abolishes the phenotype. The hormone response is inhibited by the β-blocker propranolol. We provide mechanistic insights into the basis of neuroendocrine hormone-mediated haemolysis by S. typhi, increasing our understanding of inter-kingdom signalling.

AB - Salmonella enterica serovar Typhi (S. typhi) causes typhoid fever. We show that exposure of S. typhi to neuroendocrine stress hormones results in haemolysis, which is associated with the release of haemolysin E in membrane vesicles. This effect is attributed to increased expression of the small RNA micA and RNA chaperone Hfq, with concomitant downregulation of outer membrane protein A. Deletion of micA or the two-component signal-transduction system, CpxAR, abolishes the phenotype. The hormone response is inhibited by the β-blocker propranolol. We provide mechanistic insights into the basis of neuroendocrine hormone-mediated haemolysis by S. typhi, increasing our understanding of inter-kingdom signalling.

KW - Gene Expression Regulation, Bacterial

KW - Salmonella typhi

KW - Hormones

KW - Bacterial Proteins

KW - Down-Regulation

KW - Propranolol

KW - Norepinephrine

KW - Hemolysis

KW - Protein Kinases

KW - Hemolysin Proteins

KW - Bacterial Outer Membrane Proteins

KW - Epinephrine

KW - Signal Transduction

KW - Stress, Physiological

U2 - 10.1038/embor.2011.4

DO - 10.1038/embor.2011.4

M3 - Article

C2 - 21331094

SN - 1469-221X

VL - 12

SP - 252

EP - 258

JO - EMBO Reports

JF - EMBO Reports

IS - 3

ER -

Salmonella Typhi sense host neuroendocrine stress hormones and release the toxin haemolysin E

Abstract

Keywords / Materials (for Non-textual outputs)

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