SARS-CoV-2 Causes a Significant Stress Response Mediated by Small RNAs in the Blood of COVID-19 Patients

Xi Liu; Yan-Zi Wen; Zi-Liang Huang; Xia Shen; Jun-Hao Wang; Yi-Hai Luo; Wen-Xin Chen; Zhao-Rong Lun; Hui-Bin Li; Liang-Hu Qu; Hong Shan; Ling-Ling Zheng

doi:10.1016/j.omtn.2021.12.034

SARS-CoV-2 Causes a Significant Stress Response Mediated by Small RNAs in the Blood of COVID-19 Patients

Xi Liu, Yan-Zi Wen, Zi-Liang Huang, Xia Shen, Jun-Hao Wang, Yi-Hai Luo, Wen-Xin Chen, Zhao-Rong Lun, Hui-Bin Li, Liang-Hu Qu, Hong Shan, Ling-Ling Zheng

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Abstract / Description of output

SARS-CoV-2 has had a serious impact on the world. In this study, small RNAs from the blood of COVID-19 patients with moderate or severe symptoms were extracted for high-throughput sequencing and analysis. Interestingly, the levels of a special group of tRNA-derived small RNAs (tsRNAs) were found to be dramatically upregulated after SARS-CoV-2 infection, particularly in COVID-19 patients with severe symptoms. In particular, the 3'CCA tsRNAs from Gly-tRNA were highly consistent with the inflammation indicator C-reactive protein (CRP). In addition, we found that the majority of significantly changed microRNAs (miRNAs) were associated with endoplasmic reticulum (ER)/unfolded protein response (UPR) sensors, which may lead to the induction of proinflammatory cytokine and immune responses. This study found that SARS-CoV-2 infection caused significant changes in the levels of stress-associated small RNAs in patient blood and their potential functions. Our research revealed that the cells of COVID-19 patients undergo tremendous stress and respond, which can be reflected or regulated by sncRNAs, thus providing potential thought for therapeutic intervention in COVID-19 by modulating small RNA levels or activities.

Original language	English
Journal	Molecular Therapy - Nucleic Acids
Early online date	31 Dec 2021
DOIs	https://doi.org/10.1016/j.omtn.2021.12.034
Publication status	E-pub ahead of print - 31 Dec 2021

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SARS-CoV-2 Causes a Significant Stress Response Mediated by Small RNAs in the Blood of COVID-19 PatientsAccepted author manuscript, 8.59 MB

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title = "SARS-CoV-2 Causes a Significant Stress Response Mediated by Small RNAs in the Blood of COVID-19 Patients",

abstract = "SARS-CoV-2 has had a serious impact on the world. In this study, small RNAs from the blood of COVID-19 patients with moderate or severe symptoms were extracted for high-throughput sequencing and analysis. Interestingly, the levels of a special group of tRNA-derived small RNAs (tsRNAs) were found to be dramatically upregulated after SARS-CoV-2 infection, particularly in COVID-19 patients with severe symptoms. In particular, the 3'CCA tsRNAs from Gly-tRNA were highly consistent with the inflammation indicator C-reactive protein (CRP). In addition, we found that the majority of significantly changed microRNAs (miRNAs) were associated with endoplasmic reticulum (ER)/unfolded protein response (UPR) sensors, which may lead to the induction of proinflammatory cytokine and immune responses. This study found that SARS-CoV-2 infection caused significant changes in the levels of stress-associated small RNAs in patient blood and their potential functions. Our research revealed that the cells of COVID-19 patients undergo tremendous stress and respond, which can be reflected or regulated by sncRNAs, thus providing potential thought for therapeutic intervention in COVID-19 by modulating small RNA levels or activities.",

author = "Xi Liu and Yan-Zi Wen and Zi-Liang Huang and Xia Shen and Jun-Hao Wang and Yi-Hai Luo and Wen-Xin Chen and Zhao-Rong Lun and Hui-Bin Li and Liang-Hu Qu and Hong Shan and Ling-Ling Zheng",

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T1 - SARS-CoV-2 Causes a Significant Stress Response Mediated by Small RNAs in the Blood of COVID-19 Patients

AU - Liu, Xi

AU - Wen, Yan-Zi

AU - Huang, Zi-Liang

AU - Shen, Xia

AU - Wang, Jun-Hao

AU - Luo, Yi-Hai

AU - Chen, Wen-Xin

AU - Lun, Zhao-Rong

AU - Li, Hui-Bin

AU - Qu, Liang-Hu

AU - Shan, Hong

AU - Zheng, Ling-Ling

PY - 2021/12/31

Y1 - 2021/12/31

N2 - SARS-CoV-2 has had a serious impact on the world. In this study, small RNAs from the blood of COVID-19 patients with moderate or severe symptoms were extracted for high-throughput sequencing and analysis. Interestingly, the levels of a special group of tRNA-derived small RNAs (tsRNAs) were found to be dramatically upregulated after SARS-CoV-2 infection, particularly in COVID-19 patients with severe symptoms. In particular, the 3'CCA tsRNAs from Gly-tRNA were highly consistent with the inflammation indicator C-reactive protein (CRP). In addition, we found that the majority of significantly changed microRNAs (miRNAs) were associated with endoplasmic reticulum (ER)/unfolded protein response (UPR) sensors, which may lead to the induction of proinflammatory cytokine and immune responses. This study found that SARS-CoV-2 infection caused significant changes in the levels of stress-associated small RNAs in patient blood and their potential functions. Our research revealed that the cells of COVID-19 patients undergo tremendous stress and respond, which can be reflected or regulated by sncRNAs, thus providing potential thought for therapeutic intervention in COVID-19 by modulating small RNA levels or activities.

AB - SARS-CoV-2 has had a serious impact on the world. In this study, small RNAs from the blood of COVID-19 patients with moderate or severe symptoms were extracted for high-throughput sequencing and analysis. Interestingly, the levels of a special group of tRNA-derived small RNAs (tsRNAs) were found to be dramatically upregulated after SARS-CoV-2 infection, particularly in COVID-19 patients with severe symptoms. In particular, the 3'CCA tsRNAs from Gly-tRNA were highly consistent with the inflammation indicator C-reactive protein (CRP). In addition, we found that the majority of significantly changed microRNAs (miRNAs) were associated with endoplasmic reticulum (ER)/unfolded protein response (UPR) sensors, which may lead to the induction of proinflammatory cytokine and immune responses. This study found that SARS-CoV-2 infection caused significant changes in the levels of stress-associated small RNAs in patient blood and their potential functions. Our research revealed that the cells of COVID-19 patients undergo tremendous stress and respond, which can be reflected or regulated by sncRNAs, thus providing potential thought for therapeutic intervention in COVID-19 by modulating small RNA levels or activities.

U2 - 10.1016/j.omtn.2021.12.034

DO - 10.1016/j.omtn.2021.12.034

M3 - Article

C2 - 35003892

SN - 2162-2531

JO - Molecular Therapy - Nucleic Acids

JF - Molecular Therapy - Nucleic Acids

ER -

SARS-CoV-2 Causes a Significant Stress Response Mediated by Small RNAs in the Blood of COVID-19 Patients

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