TY - JOUR
T1 - SARS-CoV-2 RNA levels in Scotland’s wastewater
AU - Scorza, Livia
AU - Cameron, Graeme J.
AU - Murray Williams, Roisin
AU - Findlay, David
AU - Bolland, Julie
AU - Cerghizan, Brindusa
AU - Campbell, Kirsty
AU - Thomson, David
AU - Corbishley, Alexander
AU - Gally, David
AU - Fitzgerald, Stephen
AU - Low, Alison
AU - McAteer, Sean
AU - Roberts, Adrian
AU - Fang, Zhou
AU - Mayer, Claus Dieter
AU - Frantsuzova, Anastasia
AU - Baby, Sumy V
AU - Zielinski, Tomasz
AU - Millar, Andrew J
N1 - Funding Information:
We thank Scottish Water for providing all samples used in this project. This work was funded by Scotland’s Centre of Expertise for Waters (CREW; Grant CD2021_03 Methodology for the detection of new variants of SARS-CoV-2 in wastewater) and by Scottish Environment Protection Agency (SEPA).
Funding Information:
We thank Scottish Water for providing all samples used in this project. This work was funded by Scotland’s Centre of Expertise for Waters (CREW; Grant CD2021_03 Methodology for the detection of new variants of SARS-CoV-2 in wastewater) and by Scottish Environment Protection Agency (SEPA).
Publisher Copyright:
© 2022, The Author(s).
PY - 2022/11/18
Y1 - 2022/11/18
N2 - Nationwide, wastewater-based monitoring was newly established in Scotland to track the levels of SARS-CoV-2 viral RNA shed into the sewage network, during the COVID-19 pandemic. We present a curated, reference dataset produced by this national programme, from May 2020 to February 2022. Viral levels were analysed by RT-qPCR assays of the N1 gene, on RNA extracted from wastewater sampled at 162 locations. Locations were sampled up to four times per week, typically once or twice per week, and in response to local needs. We report sampling site locations with geographical coordinates, the total population in the catchment for each site, and the information necessary for data normalisation, such as the incoming wastewater flow values and ammonia concentration, when these were available. The methodology for viral quantification and data analysis is briefly described, with links to detailed protocols online. These wastewater data are contributing to estimates of disease prevalence and the viral reproduction number (R) in Scotland and in the UK.
AB - Nationwide, wastewater-based monitoring was newly established in Scotland to track the levels of SARS-CoV-2 viral RNA shed into the sewage network, during the COVID-19 pandemic. We present a curated, reference dataset produced by this national programme, from May 2020 to February 2022. Viral levels were analysed by RT-qPCR assays of the N1 gene, on RNA extracted from wastewater sampled at 162 locations. Locations were sampled up to four times per week, typically once or twice per week, and in response to local needs. We report sampling site locations with geographical coordinates, the total population in the catchment for each site, and the information necessary for data normalisation, such as the incoming wastewater flow values and ammonia concentration, when these were available. The methodology for viral quantification and data analysis is briefly described, with links to detailed protocols online. These wastewater data are contributing to estimates of disease prevalence and the viral reproduction number (R) in Scotland and in the UK.
UR - https://github.com/BioRDM/COVID-Wastewater-Scotland
U2 - 10.1038/s41597-022-01788-3
DO - 10.1038/s41597-022-01788-3
M3 - Article
VL - 9
JO - Scientific Data
JF - Scientific Data
SN - 2052-4463
IS - 713
M1 - 713
ER -