SbcCD regulation and localization in Escherichia coli

Elise Darmon; Manuel A Lopez-Vernaza; Anne C Helness; Amanda Borking; Emily Wilson; Zubin Thacker; Laura Wardrope; David R F Leach

doi:10.1128/JB.00489-07

SbcCD regulation and localization in Escherichia coli

Elise Darmon, Manuel A Lopez-Vernaza, Anne C Helness, Amanda Borking, Emily Wilson, Zubin Thacker, Laura Wardrope, David R F Leach

School of Biological Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

The SbcCD complex and its homologues play important roles in DNA repair and in the maintenance of genome stability. In Escherichia coli, the in vitro functions of SbcCD have been well characterized, but its exact cellular role remains elusive. This work investigates the regulation of the sbcDC operon and the cellular localization of the SbcC and SbcD proteins. Transcription of the sbcDC operon is shown to be dependent on starvation and RpoS protein. Overexpressed SbcC protein forms foci that colocalize with the replication factory, while overexpressed SbcD protein is distributed through the cytoplasm.

Original language	English
Pages (from-to)	6686-94
Number of pages	9
Journal	Journal of Bacteriology
Volume	189
Issue number	18
DOIs	https://doi.org/10.1128/JB.00489-07
Publication status	Published - 2007

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AU - Darmon, Elise

AU - Lopez-Vernaza, Manuel A

AU - Helness, Anne C

AU - Borking, Amanda

AU - Wilson, Emily

AU - Thacker, Zubin

AU - Wardrope, Laura

AU - Leach, David R F

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AB - The SbcCD complex and its homologues play important roles in DNA repair and in the maintenance of genome stability. In Escherichia coli, the in vitro functions of SbcCD have been well characterized, but its exact cellular role remains elusive. This work investigates the regulation of the sbcDC operon and the cellular localization of the SbcC and SbcD proteins. Transcription of the sbcDC operon is shown to be dependent on starvation and RpoS protein. Overexpressed SbcC protein forms foci that colocalize with the replication factory, while overexpressed SbcD protein is distributed through the cytoplasm.

U2 - 10.1128/JB.00489-07

DO - 10.1128/JB.00489-07

M3 - Article

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VL - 189

SP - 6686

EP - 6694

JO - Journal of Bacteriology

JF - Journal of Bacteriology

SN - 0021-9193

IS - 18

ER -

SbcCD regulation and localization in Escherichia coli

Abstract

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