SCOTfluors: Small, Conjugatable, Orthogonal and Tunable Fluorophores for in vivo Imaging of Cell Metabolism

Sam Benson; Antonio Fernandez; Nicole D. Barth; Fabio De Moliner; Mathew H. Horrocks; C. Simon Herrington; Jose Luis Abad; Antonio  Delgado; Lisa Kelly; Ziyuan Chang; Yi Feng; Miyako Nishiura; Yuichiro  Hori; Kazuya  Kikuchi; Marc Vendrell

doi:10.1002/ange.201900465

SCOTfluors: Small, Conjugatable, Orthogonal and Tunable Fluorophores for in vivo Imaging of Cell Metabolism

Sam Benson, Antonio Fernandez, Nicole D. Barth, Fabio De Moliner, Mathew H. Horrocks, C. Simon Herrington, Jose Luis Abad, Antonio Delgado, Lisa Kelly, Ziyuan Chang, Yi Feng, Miyako Nishiura, Yuichiro Hori, Kazuya Kikuchi, Marc Vendrell

Research output: Contribution to journal › Article › peer-review

Abstract

The transport and trafficking of metabolites are critical for the correct functioning of live cells. However, in situ metabolic imaging studies are hampered by the lack of fluorescent chemical structures that allow direct monitoring of small metabolites under physiological conditions with high spatial and temporal resolution. Herein we describe SCOTfluors as novel small-sized multi-colored fluorophores for real-time tracking of essential metabolites in live cells and in vivo and for the acquisition of metabolic profiles from human cancer cells of variable origin.

Original language	English
Pages (from-to)	6985-6989
Journal	Angewandte Chemie
Volume	131
Issue number	21
Early online date	28 Mar 2019
DOIs	https://doi.org/10.1002/ange.201900465
Publication status	Published - 20 May 2019

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10.1002/ange.201900465

Benson_et_al-2019-Angewandte_Chemie_International_EditionFinal published version, 2.11 MB

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Dynamic Activatable Fluorophores
Vendrell Escobar, M.
EU government bodies
1/06/18 → 31/05/23
Project: Research
Functional imaging probes for GLUT4 localization
Vendrell Escobar, M.
UK-based charities
16/03/18 → 15/03/22
Project: Research
Fellowship for Antonio Fernandez. FLUORODRUGS: Smart Theranostic Agents for the Tumour Microenvironment
Vendrell Escobar, M.
EU government bodies
1/01/17 → 31/12/18
Project: Research

C Simon Herrington
- simon.herringtoned.acuk
- Deanery of Molecular, Genetic and Population Health Sciences - Chair of Molecular Pathology of Cancer
- Edinburgh Pathology
- Edinburgh Cancer Research Centre
Person: Academic: Research Active
Mathew Horrocks
- School of Chemistry - Lecturer in Biophysical Chemistry
Person: Academic: Research Active
Marc Vendrell Escobar
- marc.vendrelled.acuk
- mvendrelstaffmail.ed.acuk
- Deanery of Clinical Sciences - Personal Chair Translational Chemistry & Biomedical Imaging
- Centre for Inflammation Research
Person: Academic: Research Active

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Benson, S., Fernandez, A., Barth, N. D., De Moliner, F., Horrocks, M. H., Herrington, C. S., Abad, J. L., Delgado, A., Kelly, L., Chang, Z., Feng, Y., Nishiura, M., Hori, Y., Kikuchi, K., & Vendrell, M. (2019). SCOTfluors: Small, Conjugatable, Orthogonal and Tunable Fluorophores for in vivo Imaging of Cell Metabolism. Angewandte Chemie, 131(21), 6985-6989. https://doi.org/10.1002/ange.201900465

Benson, Sam ; Fernandez, Antonio ; Barth, Nicole D. ; De Moliner, Fabio ; Horrocks, Mathew H. ; Herrington, C. Simon ; Abad, Jose Luis ; Delgado, Antonio ; Kelly, Lisa ; Chang, Ziyuan ; Feng, Yi ; Nishiura, Miyako ; Hori, Yuichiro ; Kikuchi, Kazuya ; Vendrell, Marc. / SCOTfluors: Small, Conjugatable, Orthogonal and Tunable Fluorophores for in vivo Imaging of Cell Metabolism. In: Angewandte Chemie. 2019 ; Vol. 131, No. 21. pp. 6985-6989.

@article{2c3ce847896f4cddac3738b9a6149b38,

title = "SCOTfluors: Small, Conjugatable, Orthogonal and Tunable Fluorophores for in vivo Imaging of Cell Metabolism",

abstract = "The transport and trafficking of metabolites are critical for the correct functioning of live cells. However, in situ metabolic imaging studies are hampered by the lack of fluorescent chemical structures that allow direct monitoring of small metabolites under physiological conditions with high spatial and temporal resolution. Herein we describe SCOTfluors as novel small-sized multi-colored fluorophores for real-time tracking of essential metabolites in live cells and in vivo and for the acquisition of metabolic profiles from human cancer cells of variable origin.",

author = "Sam Benson and Antonio Fernandez and Barth, {Nicole D.} and {De Moliner}, Fabio and Horrocks, {Mathew H.} and Herrington, {C. Simon} and Abad, {Jose Luis} and Antonio Delgado and Lisa Kelly and Ziyuan Chang and Yi Feng and Miyako Nishiura and Yuichiro Hori and Kazuya Kikuchi and Marc Vendrell",

year = "2019",

month = may,

day = "20",

doi = "10.1002/ange.201900465",

language = "English",

volume = "131",

pages = "6985--6989",

journal = "Angewandte Chemie",

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publisher = "John Wiley & Sons Inc.",

number = "21",

}

Benson, S, Fernandez, A, Barth, ND, De Moliner, F , Horrocks, MH , Herrington, CS, Abad, JL, Delgado, A, Kelly, L, Chang, Z , Feng, Y, Nishiura, M, Hori, Y, Kikuchi, K & Vendrell, M 2019, 'SCOTfluors: Small, Conjugatable, Orthogonal and Tunable Fluorophores for in vivo Imaging of Cell Metabolism', Angewandte Chemie, vol. 131, no. 21, pp. 6985-6989. https://doi.org/10.1002/ange.201900465

SCOTfluors: Small, Conjugatable, Orthogonal and Tunable Fluorophores for in vivo Imaging of Cell Metabolism. / Benson, Sam; Fernandez, Antonio; Barth, Nicole D.; De Moliner, Fabio ; Horrocks, Mathew H.; Herrington, C. Simon; Abad, Jose Luis; Delgado, Antonio ; Kelly, Lisa; Chang, Ziyuan ; Feng, Yi; Nishiura, Miyako; Hori, Yuichiro ; Kikuchi, Kazuya ; Vendrell, Marc.

In: Angewandte Chemie, Vol. 131, No. 21, 20.05.2019, p. 6985-6989.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - SCOTfluors: Small, Conjugatable, Orthogonal and Tunable Fluorophores for in vivo Imaging of Cell Metabolism

AU - Benson, Sam

AU - Fernandez, Antonio

AU - Barth, Nicole D.

AU - De Moliner, Fabio

AU - Horrocks, Mathew H.

AU - Herrington, C. Simon

AU - Abad, Jose Luis

AU - Delgado, Antonio

AU - Kelly, Lisa

AU - Chang, Ziyuan

AU - Feng, Yi

AU - Nishiura, Miyako

AU - Hori, Yuichiro

AU - Kikuchi, Kazuya

AU - Vendrell, Marc

PY - 2019/5/20

Y1 - 2019/5/20

N2 - The transport and trafficking of metabolites are critical for the correct functioning of live cells. However, in situ metabolic imaging studies are hampered by the lack of fluorescent chemical structures that allow direct monitoring of small metabolites under physiological conditions with high spatial and temporal resolution. Herein we describe SCOTfluors as novel small-sized multi-colored fluorophores for real-time tracking of essential metabolites in live cells and in vivo and for the acquisition of metabolic profiles from human cancer cells of variable origin.

AB - The transport and trafficking of metabolites are critical for the correct functioning of live cells. However, in situ metabolic imaging studies are hampered by the lack of fluorescent chemical structures that allow direct monitoring of small metabolites under physiological conditions with high spatial and temporal resolution. Herein we describe SCOTfluors as novel small-sized multi-colored fluorophores for real-time tracking of essential metabolites in live cells and in vivo and for the acquisition of metabolic profiles from human cancer cells of variable origin.

U2 - 10.1002/ange.201900465

DO - 10.1002/ange.201900465

M3 - Article

VL - 131

SP - 6985

EP - 6989

JO - Angewandte Chemie

JF - Angewandte Chemie

SN - 0044-8249

IS - 21

ER -

SCOTfluors: Small, Conjugatable, Orthogonal and Tunable Fluorophores for in vivo Imaging of Cell Metabolism

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Dynamic Activatable Fluorophores

Functional imaging probes for GLUT4 localization

Fellowship for Antonio Fernandez. FLUORODRUGS: Smart Theranostic Agents for the Tumour Microenvironment

Profiles

C Simon Herrington

Mathew Horrocks

Marc Vendrell Escobar

Cite this