TY - JOUR
T1 - Screening great ape museum specimens for DNA viruses
AU - Hämmerle, Michelle
AU - Guellil, Meriam
AU - Trgovec-Greif, Lovro
AU - Cheronet, Olivia
AU - Sawyer, Susanna
AU - Ruiz-Gartzia, Irune
AU - Lizano, Esther
AU - Rymbekova, Aigerim
AU - Gelabert, Pere
AU - Bernardi, Paolo
AU - Han, Sojung
AU - Rattei, Thomas
AU - Schuenemann, Verena J.
AU - Marques-Bonet, Tomas
AU - Guschanski, Katerina
AU - Calvignac-Spencer, Sebastien
AU - Pinhasi, Ron
AU - Kuhlwilm, Martin
N1 - The computational results of this work have been achieved using the Life Science Compute Cluster (LiSC) of the University of Vienna. We are grateful to the Zoologisches Forschungsmuseum A. Koenig, Leibniz-Institut zur Analyse des Biodiversitätswandels in Bonn, in particular Eva Bärmann, Jan Decher, and Christian Montermann at the Section Theriology; to Irina Ruf and Katrin Krohmann at the Mammalogy collection at Senckenberg Forschungsinstitut und Naturmuseum Frankfurt/M.; to Frank Zachos and Alexander Bibl at Naturhistorisches Museum Wien; to Stefan Merker at Zoology at Staatliches Museum für Naturkunde Stuttgart; to Petr Benda at Department of Zoology at National Museum (Natural History) Prague; to Clara Stefen and Jens Jakobitz at Mammalogie at Senckenberg Naturhistorische Sammlungen Dresden; to Robert Lindner at Haus der Natur – Museum für Natur und Technik in Salzburg; to Christiane Funk and Frieder Mayer at the mammalian collection at Museum für Naturkunde/Leibniz-Institut für Evolutions- und Biodiversitätsforschung in Berlin
PY - 2024/11/30
Y1 - 2024/11/30
N2 - Natural history museum collections harbour a record of wild species from the past centuries, providing a unique opportunity to study animals as well as their infectious agents. Thousands of great ape specimens are kept in these collections, and could become an important resource for studying the evolution of DNA viruses. Their genetic material is likely to be preserved in dry museum specimens, as reported previously for monkeypox virus genomes from historical orangutan specimens. Here, we screened 209 great ape museum specimens for 99 different DNA viruses, using hybridization capture coupled with short-read high-throughput sequencing. We determined the presence of multiple viruses within this dataset from historical specimens and obtained several near-complete viral genomes. In particular, we report high-coverage (>18-fold) hepatitis B virus genomes from one gorilla and two chimpanzee individuals, which are phylogenetically placed within clades infecting the respective host species.
AB - Natural history museum collections harbour a record of wild species from the past centuries, providing a unique opportunity to study animals as well as their infectious agents. Thousands of great ape specimens are kept in these collections, and could become an important resource for studying the evolution of DNA viruses. Their genetic material is likely to be preserved in dry museum specimens, as reported previously for monkeypox virus genomes from historical orangutan specimens. Here, we screened 209 great ape museum specimens for 99 different DNA viruses, using hybridization capture coupled with short-read high-throughput sequencing. We determined the presence of multiple viruses within this dataset from historical specimens and obtained several near-complete viral genomes. In particular, we report high-coverage (>18-fold) hepatitis B virus genomes from one gorilla and two chimpanzee individuals, which are phylogenetically placed within clades infecting the respective host species.
KW - Museomics
KW - Great Apes
KW - Target-enrichment capture
KW - Viruses
KW - Hepatitis B virus
UR - https://github.com/admixVIE/Great-Ape-DNA-Virome
UR - https://www.ncbi.nlm.nih.gov/sra?term=PRJEB75038&cmd=DetailsSearch
U2 - 10.1038/s41598-024-80780-w
DO - 10.1038/s41598-024-80780-w
M3 - Article
SN - 2045-2322
VL - 14
JO - Scientific Reports
JF - Scientific Reports
IS - 1
M1 - 29806
ER -