Screening of xenobiotic compounds degrading microorganisms using biosensor techniques

B Beyersdorf-Radeck, K Riedel, U Karlson, Till Bachmann, R D Schmid

Research output: Contribution to journalArticlepeer-review

Abstract

A screening device based on microorganisms immobilised onto a Clark-type oxygen electrode was used to monitor the potential of these microorganisms for the degradation and detection of xenobiotic compounds especially their chlorinated derivatives. The sensitivity and specificity of various species of Pseudomonas, Sphinomonas, Ralstonia, Rhodococcus were characterised in relation to xenobiotic compounds by using biosensor techniques. The following groups of xenobiotics were subjects of investigation: chlorophenols, chlorobenzoates, 2,4-D, PCB, dibenzofurane and their putative intermediates. Using this simple setup it proved possible to screen microbial strains for their potential to catabolize aromatic and chloroaromatic compounds under oxygen consumption. In a kinetic regime, a reproducible signal was obtained within minutes. Based on these results the sensor technique was a suitable method for the rapid characterization of microorganisms and allowed to gather information about the substrate spectrum.
Original languageEnglish
Pages (from-to)239-45
Number of pages7
JournalMicrobiological research
Volume153
Issue number3
Publication statusPublished - 1998

Keywords

  • 2,4-Dichlorophenoxyacetic Acid
  • Bacteria
  • Benzofurans
  • Biosensing Techniques
  • Chlorine Compounds
  • Chlorobenzoates
  • Chlorophenols
  • Cupriavidus necator
  • Polychlorinated Biphenyls
  • Pseudomonas
  • Rhodococcus
  • Xenobiotics

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