Screening trematodes for novel intervention targets: a proteomic and immunological comparison of Schistosoma haematobium, Schistosoma bovis and Echinostoma caproni

Melissa Higon, Graeme Cowan, Norman Nausch, David Cavanagh, Ana Oleaga, Rafael Toledo, J. Russell Stothard, Oreto Antunez, Antonio Marcilla, Richard Burchmore, Francisca Mutapi*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

With the current paucity of vaccine targets for parasitic diseases, particularly those in childhood, the aim of this study was to compare protein expression and immune cross-reactivity between the trematodes Schistosoma haematobium, S. bovis and Echinostoma caproni in the hope of identifying novel intervention targets. Native adult parasite proteins were separated by 2-dimensional gel electrophoresis and identified through electrospray ionisation tandem mass spectrometry to produce a reference gel. Proteins from differential gel electrophoresis analyses of the three parasite proteomes were compared and screened against sera from hamsters infected with S. haematobium and E. caproni following 2-dimensional Western blotting. Differential protein expression between the three species was observed with circa 5% of proteins from S. haematobium showing expression up-regulation compared to the other two species. There was 91% similarity between the proteomes of the two Schistosoma species and 81% and 78·6% similarity between S. haematobium and S. bovis versus E. caproni, respectively. Although there were some common cross-species antigens, species-species targets were revealed which, despite evolutionary homology, could be due to phenotypic plasticity arising from different host-parasite relationships. Nevertheless, this approach helps to identify novel intervention targets which could be used as broad-spectrum candidates for future use in human and veterinary vaccines.
Original languageEnglish
Pages (from-to)1607-1619
Number of pages13
JournalParasitology
Volume138
Issue number12
DOIs
Publication statusPublished - Oct 2011

Keywords

  • Schistosoma
  • S. bovis
  • S. haematobium
  • Echinostosma caproni
  • trematode
  • proteomics
  • immunology
  • DIGE
  • DIA
  • vaccine development
  • PROTEIN DISULFIDE-ISOMERASE
  • FASCIOLA-HEPATICA
  • EXPERIMENTAL-INFECTION
  • ANCYLOSTOMA-CANINUM
  • MOLECULAR-CLONING
  • IMMUNE-RESPONSES
  • FRIEDI TREMATODA
  • GENE-EXPRESSION
  • MANSONI
  • PARASITE

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