Secretome of human aortic valves

Fernando de la Cuesta; Gloria Alvarez-Llamas; Felix Gil-Dones; Verónica M Darde; Enrique Calvo; Juan Antonio López; Fernando Vivanco; María G Barderas

doi:10.1007/978-1-62703-386-2_19

Secretome of human aortic valves

Fernando de la Cuesta, Gloria Alvarez-Llamas, Felix Gil-Dones, Verónica M Darde, Enrique Calvo, Juan Antonio López, Fernando Vivanco, María G Barderas

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract / Description of output

One of the major challenges in cardiovascular medicine is to identify candidate biomarker proteins. Between different proteomics studies, the secretome is a valuable tool in the search for biomarkers locally released by a studied tissue and remains particularly important while working with vascular tissues, since the secreted proteins would be probably shed to the blood. In this chapter, we described a method to validate the origin of secreted proteins in human aortic valves, demonstrating their synthesis and release by the tissue and ruling out blood origin.

Original language	English
Title of host publication	Heart Proteomics
Pages	237-43
Number of pages	7
Volume	1005
DOIs	https://doi.org/10.1007/978-1-62703-386-2_19
Publication status	Published - 2013

Keywords / Materials (for Non-textual outputs)

Aortic Valve
Aortic Valve Stenosis
Chromatography, Liquid
Culture Media, Conditioned
Humans
Peptide Fragments
Peptide Mapping
Proteolysis
Proteome
Tandem Mass Spectrometry
Tissue Culture Techniques
Trypsin

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10.1007/978-1-62703-386-2_19

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title = "Secretome of human aortic valves",

abstract = "One of the major challenges in cardiovascular medicine is to identify candidate biomarker proteins. Between different proteomics studies, the secretome is a valuable tool in the search for biomarkers locally released by a studied tissue and remains particularly important while working with vascular tissues, since the secreted proteins would be probably shed to the blood. In this chapter, we described a method to validate the origin of secreted proteins in human aortic valves, demonstrating their synthesis and release by the tissue and ruling out blood origin.",

keywords = "Aortic Valve, Aortic Valve Stenosis, Chromatography, Liquid, Culture Media, Conditioned, Humans, Peptide Fragments, Peptide Mapping, Proteolysis, Proteome, Tandem Mass Spectrometry, Tissue Culture Techniques, Trypsin",

author = "{de la Cuesta}, Fernando and Gloria Alvarez-Llamas and Felix Gil-Dones and Darde, {Ver{\'o}nica M} and Enrique Calvo and L{\'o}pez, {Juan Antonio} and Fernando Vivanco and Barderas, {Mar{\'i}a G}",

year = "2013",

doi = "10.1007/978-1-62703-386-2_19",

language = "English",

volume = "1005",

pages = "237--43",

booktitle = "Heart Proteomics",

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TY - CHAP

T1 - Secretome of human aortic valves

AU - de la Cuesta, Fernando

AU - Alvarez-Llamas, Gloria

AU - Gil-Dones, Felix

AU - Darde, Verónica M

AU - Calvo, Enrique

AU - López, Juan Antonio

AU - Vivanco, Fernando

AU - Barderas, María G

PY - 2013

Y1 - 2013

N2 - One of the major challenges in cardiovascular medicine is to identify candidate biomarker proteins. Between different proteomics studies, the secretome is a valuable tool in the search for biomarkers locally released by a studied tissue and remains particularly important while working with vascular tissues, since the secreted proteins would be probably shed to the blood. In this chapter, we described a method to validate the origin of secreted proteins in human aortic valves, demonstrating their synthesis and release by the tissue and ruling out blood origin.

AB - One of the major challenges in cardiovascular medicine is to identify candidate biomarker proteins. Between different proteomics studies, the secretome is a valuable tool in the search for biomarkers locally released by a studied tissue and remains particularly important while working with vascular tissues, since the secreted proteins would be probably shed to the blood. In this chapter, we described a method to validate the origin of secreted proteins in human aortic valves, demonstrating their synthesis and release by the tissue and ruling out blood origin.

KW - Aortic Valve

KW - Aortic Valve Stenosis

KW - Chromatography, Liquid

KW - Culture Media, Conditioned

KW - Humans

KW - Peptide Fragments

KW - Peptide Mapping

KW - Proteolysis

KW - Proteome

KW - Tandem Mass Spectrometry

KW - Tissue Culture Techniques

KW - Trypsin

U2 - 10.1007/978-1-62703-386-2_19

DO - 10.1007/978-1-62703-386-2_19

M3 - Chapter

C2 - 23606262

VL - 1005

SP - 237

EP - 243

BT - Heart Proteomics

ER -

Secretome of human aortic valves

Abstract / Description of output

Keywords / Materials (for Non-textual outputs)

Access to Document

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