Seeding of YACs over regions 1q41-q42.3 and 11q14.3-q23 with microdissection clones

J Petit; P Boisseau; K Evans; S Christie; C Gosden; W Muir; D St Clair; D Porteous; B Arveiler

Seeding of YACs over regions 1q41-q42.3 and 11q14.3-q23 with microdissection clones

J Petit, P Boisseau, K Evans, S Christie, C Gosden, W Muir, D St Clair, D Porteous, B Arveiler

Research output: Contribution to journal › Article › peer-review

Abstract

We describe the use of pooled, region-specific hybridisation probes to screen high-density replica filters of a human genome YAC library. The probes were derived by microdissection of an approximately 30-Mbp region subtending the translocation breakpoint on a der(1)(1;11)(q42.1;q14.3) chromosome. Of 70 microdissection clones used in pools of 4-10, 47 identified a total of 77 YAC recombinants, representing over 50% of the microdissected region. This strategy can easily be adapted to other poorly mapped subchromosomal regions of the human or other mammalian genomes and will provide a solid framework for detailed contig map constructions.

Original language	English
Pages (from-to)	351-6
Number of pages	6
Journal	European Journal of Human Genetics
Volume	3
Issue number	6
Publication status	Published - 1995

Keywords

Chromosomes, Artificial, Yeast
Chromosomes, Human, Pair 1
Chromosomes, Human, Pair 11
DNA Transposable Elements
Gene Library
Genetic Testing
Humans
Repetitive Sequences, Nucleic Acid

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@article{879996caae9241d5bf03675c96f3b96e,

title = "Seeding of YACs over regions 1q41-q42.3 and 11q14.3-q23 with microdissection clones",

abstract = "We describe the use of pooled, region-specific hybridisation probes to screen high-density replica filters of a human genome YAC library. The probes were derived by microdissection of an approximately 30-Mbp region subtending the translocation breakpoint on a der(1)(1;11)(q42.1;q14.3) chromosome. Of 70 microdissection clones used in pools of 4-10, 47 identified a total of 77 YAC recombinants, representing over 50% of the microdissected region. This strategy can easily be adapted to other poorly mapped subchromosomal regions of the human or other mammalian genomes and will provide a solid framework for detailed contig map constructions.",

keywords = "Chromosomes, Artificial, Yeast, Chromosomes, Human, Pair 1, Chromosomes, Human, Pair 11, DNA Transposable Elements, Gene Library, Genetic Testing, Humans, Repetitive Sequences, Nucleic Acid",

author = "J Petit and P Boisseau and K Evans and S Christie and C Gosden and W Muir and {St Clair}, D and D Porteous and B Arveiler",

year = "1995",

language = "English",

volume = "3",

pages = "351--6",

journal = "European Journal of Human Genetics",

issn = "1018-4813",

publisher = "Springer Nature",

number = "6",

}

TY - JOUR

T1 - Seeding of YACs over regions 1q41-q42.3 and 11q14.3-q23 with microdissection clones

AU - Petit, J

AU - Boisseau, P

AU - Evans, K

AU - Christie, S

AU - Gosden, C

AU - Muir, W

AU - St Clair, D

AU - Porteous, D

AU - Arveiler, B

PY - 1995

Y1 - 1995

N2 - We describe the use of pooled, region-specific hybridisation probes to screen high-density replica filters of a human genome YAC library. The probes were derived by microdissection of an approximately 30-Mbp region subtending the translocation breakpoint on a der(1)(1;11)(q42.1;q14.3) chromosome. Of 70 microdissection clones used in pools of 4-10, 47 identified a total of 77 YAC recombinants, representing over 50% of the microdissected region. This strategy can easily be adapted to other poorly mapped subchromosomal regions of the human or other mammalian genomes and will provide a solid framework for detailed contig map constructions.

AB - We describe the use of pooled, region-specific hybridisation probes to screen high-density replica filters of a human genome YAC library. The probes were derived by microdissection of an approximately 30-Mbp region subtending the translocation breakpoint on a der(1)(1;11)(q42.1;q14.3) chromosome. Of 70 microdissection clones used in pools of 4-10, 47 identified a total of 77 YAC recombinants, representing over 50% of the microdissected region. This strategy can easily be adapted to other poorly mapped subchromosomal regions of the human or other mammalian genomes and will provide a solid framework for detailed contig map constructions.

KW - Chromosomes, Artificial, Yeast

KW - Chromosomes, Human, Pair 1

KW - Chromosomes, Human, Pair 11

KW - DNA Transposable Elements

KW - Gene Library

KW - Genetic Testing

KW - Humans

KW - Repetitive Sequences, Nucleic Acid

M3 - Article

C2 - 8825577

VL - 3

SP - 351

EP - 356

JO - European Journal of Human Genetics

JF - European Journal of Human Genetics

SN - 1018-4813

IS - 6

ER -