Seeding of YACs over regions 1q41-q42.3 and 11q14.3-q23 with microdissection clones

J Petit, P Boisseau, K Evans, S Christie, C Gosden, W Muir, D St Clair, D Porteous, B Arveiler

Research output: Contribution to journalArticlepeer-review

Abstract

We describe the use of pooled, region-specific hybridisation probes to screen high-density replica filters of a human genome YAC library. The probes were derived by microdissection of an approximately 30-Mbp region subtending the translocation breakpoint on a der(1)(1;11)(q42.1;q14.3) chromosome. Of 70 microdissection clones used in pools of 4-10, 47 identified a total of 77 YAC recombinants, representing over 50% of the microdissected region. This strategy can easily be adapted to other poorly mapped subchromosomal regions of the human or other mammalian genomes and will provide a solid framework for detailed contig map constructions.
Original languageEnglish
Pages (from-to)351-6
Number of pages6
JournalEuropean Journal of Human Genetics
Volume3
Issue number6
Publication statusPublished - 1995

Keywords

  • Chromosomes, Artificial, Yeast
  • Chromosomes, Human, Pair 1
  • Chromosomes, Human, Pair 11
  • DNA Transposable Elements
  • Gene Library
  • Genetic Testing
  • Humans
  • Repetitive Sequences, Nucleic Acid

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