Seh1 targets GATOR2 and Nup153 to mitotic chromosomes

Melpomeni Platani, Itaru Samejima, Kumiko Samejima, Masato T Kanemaki, W C Earnshaw

Research output: Contribution to journalArticlepeer-review

Abstract

In metazoa the Nup107-160 nucleoporin Y complex plays a major role in formation of the nuclear pore complex in interphase and is localised to kinetochores in mitosis. The Nup107-160 complex shares a single highly conserved subunit, Seh1, with the GATOR2 complex, an essential activator of mTORC1 kinase. mTORC1/GATOR2 has a central role in the coordination of cell growth and proliferation. Here we use chemical genetics and quantitative chromosome proteomics to study the role of the Seh1 protein in mitosis. Surprisingly, Seh1 is not required for the association of the Nup107-160 complex with mitotic chromosomes, but it is essential for the association of both GATOR2 complex and nucleoporin Nup153 with mitotic chromosomes. Our analysis also reveals a role for Seh1 at human centromeres, where it is required for efficient localisation of the chromosomal passenger complex (CPC). Furthermore this analysis detects a functional interaction between the Nup107-160 complex and the small kinetochore protein SKAP.

Original languageEnglish
Article numberjcs213140
Number of pages16
JournalJournal of Cell Science
Volume131
Issue number9
Early online date3 Apr 2018
DOIs
Publication statusPublished - 1 May 2018

Keywords

  • Chromosome
  • Nuclear Pore Complex (NPC)
  • mTORC
  • CPC
  • GATOR2
  • Kinetochore microtubules
  • Nup153
  • Seh1
  • mTOR

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