Projects per year
In metazoa the Nup107-160 nucleoporin Y complex plays a major role in formation of the nuclear pore complex in interphase and is localised to kinetochores in mitosis. The Nup107-160 complex shares a single highly conserved subunit, Seh1, with the GATOR2 complex, an essential activator of mTORC1 kinase. mTORC1/GATOR2 has a central role in the coordination of cell growth and proliferation. Here we use chemical genetics and quantitative chromosome proteomics to study the role of the Seh1 protein in mitosis. Surprisingly, Seh1 is not required for the association of the Nup107-160 complex with mitotic chromosomes, but it is essential for the association of both GATOR2 complex and nucleoporin Nup153 with mitotic chromosomes. Our analysis also reveals a role for Seh1 at human centromeres, where it is required for efficient localisation of the chromosomal passenger complex (CPC). Furthermore this analysis detects a functional interaction between the Nup107-160 complex and the small kinetochore protein SKAP.
- Nuclear Pore Complex (NPC)
- Kinetochore microtubules
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- 2 Finished
1/10/15 → 30/09/21
Investigation of the functional role of nucleoporins and the Nup107-160 complex in mitosis with Prof Earnshaw
1/01/09 → 30/09/12