Projects per year
Abstract / Description of output
In metazoa the Nup107-160 nucleoporin Y complex plays a major role in formation of the nuclear pore complex in interphase and is localised to kinetochores in mitosis. The Nup107-160 complex shares a single highly conserved subunit, Seh1, with the GATOR2 complex, an essential activator of mTORC1 kinase. mTORC1/GATOR2 has a central role in the coordination of cell growth and proliferation. Here we use chemical genetics and quantitative chromosome proteomics to study the role of the Seh1 protein in mitosis. Surprisingly, Seh1 is not required for the association of the Nup107-160 complex with mitotic chromosomes, but it is essential for the association of both GATOR2 complex and nucleoporin Nup153 with mitotic chromosomes. Our analysis also reveals a role for Seh1 at human centromeres, where it is required for efficient localisation of the chromosomal passenger complex (CPC). Furthermore this analysis detects a functional interaction between the Nup107-160 complex and the small kinetochore protein SKAP.
Original language | English |
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Article number | jcs213140 |
Number of pages | 16 |
Journal | Journal of Cell Science |
Volume | 131 |
Issue number | 9 |
Early online date | 3 Apr 2018 |
DOIs | |
Publication status | Published - 1 May 2018 |
Keywords / Materials (for Non-textual outputs)
- Chromosome
- Nuclear Pore Complex (NPC)
- mTORC
- CPC
- GATOR2
- Kinetochore microtubules
- Nup153
- Seh1
- mTOR
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Dive into the research topics of 'Seh1 targets GATOR2 and Nup153 to mitotic chromosomes'. Together they form a unique fingerprint.Projects
- 2 Finished
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The role of non-histone proteins in chromosome structure and function during mitosis
1/10/15 → 30/09/21
Project: Research
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Investigation of the functional role of nucleoporins and the Nup107-160 complex in mitosis with Prof Earnshaw
1/01/09 → 30/09/12
Project: Research