Selecting plasmodium falciparum infected erythrocytes for adhesion to cell lines

Yvonne Azasi*, J. Alexandra Rowe

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Plasmodium falciparum expresses variant surface antigens on the surface of mature infected erythrocytes (IEs) for binding to various receptors on host cells (cytoadhesion) to evade host immunity. This enables IEs to sequester in the microvasculature of different organs and tissues of the host, contributing to different outcomes of disease. The in vitro study of cytoadhesion involves the use of IEs and human endothelial cells or other cell lines that express host cell receptors. To enrich for IE populations that bind to certain cell types or receptors, we describe a method for panning mature pigmented trophozoite IEs on cell lines. The method enables coculturing of IEs with cells of interest and the selection of IEs that cytoadhere for continuous culturing. The method serves as a tool for generating IEs with specific cell or cell receptor adhesion phenotypes to allow detailed studies of cytoadhesion interactions.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHUMANA PRESS INC
Pages91-100
Number of pages10
Volume2470
ISBN (Electronic)978-1-0716-2189-9
ISBN (Print)978-1-0716-2188-2
DOIs
Publication statusE-pub ahead of print - 27 Jul 2022

Publication series

NameMethods in Molecular Biology
Volume2470
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • binding
  • CHO
  • cytoadhesion
  • endothelial cells
  • panning
  • plasmodium
  • selection
  • sequestration

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