@inbook{31b9a22c7cd64123a92a6038a1c05114,
title = "Selecting plasmodium falciparum infected erythrocytes for adhesion to cell lines",
abstract = "Plasmodium falciparum expresses variant surface antigens on the surface of mature infected erythrocytes (IEs) for binding to various receptors on host cells (cytoadhesion) to evade host immunity. This enables IEs to sequester in the microvasculature of different organs and tissues of the host, contributing to different outcomes of disease. The in vitro study of cytoadhesion involves the use of IEs and human endothelial cells or other cell lines that express host cell receptors. To enrich for IE populations that bind to certain cell types or receptors, we describe a method for panning mature pigmented trophozoite IEs on cell lines. The method enables coculturing of IEs with cells of interest and the selection of IEs that cytoadhere for continuous culturing. The method serves as a tool for generating IEs with specific cell or cell receptor adhesion phenotypes to allow detailed studies of cytoadhesion interactions.",
keywords = "binding, CHO, cytoadhesion, endothelial cells, panning, plasmodium, selection, sequestration",
author = "Yvonne Azasi and Rowe, {J. Alexandra}",
note = "{\textcopyright} 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2022",
month = jul,
day = "27",
doi = "10.1007/978-1-0716-2189-9_9",
language = "English",
isbn = "978-1-0716-2188-2",
volume = "2470",
series = "Methods in Molecular Biology",
publisher = "HUMANA PRESS INC",
pages = "91--100",
booktitle = "Methods in Molecular Biology",
}