Selective activation of JNK/SAPK by interleukin-1 in rabbit liver is mediated by MKK7

A Finch, P Holland, J Cooper, J Saklatvala, M Kracht

Research output: Contribution to journalArticlepeer-review

Abstract

Activation of jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) by interleukin-1 (IL-1) has been reported in many cells and in rabbit liver. Here we report selective activation of JNK/SAPK, without activation of p38 or p42 mitogen-activated protein kinases (MAPKs), by IL-1 in rabbit liver. We identified an IL-1 regulated JNK/SAPK activator present in rabbit liver using S Sepharose chromatography. It was purified and immunoprecipitated by two antisera to MAP kinase kinase 7 (MKK7). It was not recognised by an antibody to MKK4. We conclude that MKK7 is the activator of JNK/SAPK activated by IL-1 in liver and that JNK/SAPK is the only MAPK activated by IL-1 in liver.
Original languageEnglish
Pages (from-to)144-8
Number of pages5
JournalFEBS Letters
Volume418
Issue number1-2
DOIs
Publication statusPublished - 24 Nov 1997

Keywords

  • Animals
  • Immunoblotting
  • Calcium-Calmodulin-Dependent Protein Kinases
  • Enzyme Activation
  • Humans
  • Rabbits
  • Amino Acid Sequence
  • Chromatography, Ion Exchange
  • Peptide Fragments
  • Antibodies
  • p38 Mitogen-Activated Protein Kinases
  • Phosphorylation
  • Interleukin-1
  • JNK Mitogen-Activated Protein Kinases
  • MAP Kinase Kinase 7
  • Molecular Sequence Data
  • Liver
  • Protein Kinases
  • Mitogen-Activated Protein Kinases
  • Substrate Specificity
  • Mitogen-Activated Protein Kinase 1
  • Mitogen-Activated Protein Kinase Kinases

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