Abstract
Activation of jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) by interleukin-1 (IL-1) has been reported in many cells and in rabbit liver. Here we report selective activation of JNK/SAPK, without activation of p38 or p42 mitogen-activated protein kinases (MAPKs), by IL-1 in rabbit liver. We identified an IL-1 regulated JNK/SAPK activator present in rabbit liver using S Sepharose chromatography. It was purified and immunoprecipitated by two antisera to MAP kinase kinase 7 (MKK7). It was not recognised by an antibody to MKK4. We conclude that MKK7 is the activator of JNK/SAPK activated by IL-1 in liver and that JNK/SAPK is the only MAPK activated by IL-1 in liver.
| Original language | English |
|---|---|
| Pages (from-to) | 144-8 |
| Number of pages | 5 |
| Journal | FEBS Letters |
| Volume | 418 |
| Issue number | 1-2 |
| DOIs | |
| Publication status | Published - 24 Nov 1997 |
Keywords
- Animals
- Immunoblotting
- Calcium-Calmodulin-Dependent Protein Kinases
- Enzyme Activation
- Humans
- Rabbits
- Amino Acid Sequence
- Chromatography, Ion Exchange
- Peptide Fragments
- Antibodies
- p38 Mitogen-Activated Protein Kinases
- Phosphorylation
- Interleukin-1
- JNK Mitogen-Activated Protein Kinases
- MAP Kinase Kinase 7
- Molecular Sequence Data
- Liver
- Protein Kinases
- Mitogen-Activated Protein Kinases
- Substrate Specificity
- Mitogen-Activated Protein Kinase 1
- Mitogen-Activated Protein Kinase Kinases