Projects per year
Abstract / Description of output
In eukaryotes, bursts of reactive oxygen and nitrogen species mediate cellular responses to the environment by modifying cysteines of signaling proteins. Cysteine reactivity toward nitric oxide (NO) leads to formation of S-nitrosothiols (SNOs) that play important roles in pathogenesis and immunity. However, it remains poorly understood how SNOs are employed as specific, reversible signaling cues. Here we show that in plant immunity the oxidoreductase Thioredoxin-h5 (TRXh5) reverses SNO modifications by acting as a selective protein-SNO reductase. While TRXh5 failed to restore immunity in gsnor1 mutants that display excessive accumulation of the NO donor S-nitrosoglutathione, it rescued immunity in nox1 mutants that exhibit elevated levels of free NO. Rescue by TRXh5 was conferred through selective denitrosylation of excessive protein-SNO, which reinstated signaling by the immune hormone salicylic acid. Our data indicate that TRXh5 discriminates between protein-SNO substrates to provide previously unrecognized specificity and reversibility to protein-SNO signaling in plant immunity.
Original language | English |
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Pages (from-to) | 153-162 |
Number of pages | 10 |
Journal | Molecular Cell |
Volume | 56 |
Issue number | 1 |
Early online date | 4 Sept 2014 |
DOIs | |
Publication status | Published - 2 Oct 2014 |
Keywords / Materials (for Non-textual outputs)
- arabidopsis
- arabidopsis proteins
- plant immunity
- protein processing
- S-nitrosothiols
- signal transduction
- thioredoxin h
- post-translational
Fingerprint
Dive into the research topics of 'Selective protein denitrosylation activity of Thioredoxin-h5 modulates plant immunity'. Together they form a unique fingerprint.Projects
- 3 Finished
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SNO-CHIP & RED-CHIP: Novel methodologies for visualizing redox-mediated chromatin remodelling
1/05/12 → 30/04/14
Project: Research
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Redox Defence: towards a sustainable protection strategy against plant disease
1/05/11 → 30/04/12
Project: Research
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Profiles
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Steven Spoel
- School of Biological Sciences - Personal Chair of Cell Signalling and Proteostasis
Person: Academic: Research Active