Sensitive life detection strategies for low-biomass environments: optimizing extraction of nucleic acids adsorbing to terrestrial and Mars analogue minerals

Susana O. L. Direito; Andries Marees; Wilfred F. M. Roling

doi:10.1111/j.1574-6941.2012.01325.x

Sensitive life detection strategies for low-biomass environments: optimizing extraction of nucleic acids adsorbing to terrestrial and Mars analogue minerals

Susana O. L. Direito, Andries Marees, Wilfred F. M. Roling^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

The adsorption of nucleic acids to mineral matrixes can result in low extraction yields and negatively influences molecular microbial ecology studies, in particular for low-biomass environments on Earth and Mars. We determined the recovery of nucleic acids from a range of minerals relevant to Earth and Mars. Clay minerals, but also other silicates and nonsilicates, showed very low recovery (

Original language	English
Pages (from-to)	111-123
Number of pages	13
Journal	FEMS Microbiology Ecology
Volume	81
Issue number	1
DOIs	https://doi.org/10.1111/j.1574-6941.2012.01325.x
Publication status	Published - Jul 2012
Externally published	Yes

Keywords / Materials (for Non-textual outputs)

clays
DNA adsorption
DNA extraction
Mars analogue
mineralogy
subsurface
DNA EXTRACTION
QUANTITATIVE PCR
MICROBIAL-POPULATIONS
CONFORMATIONAL-CHANGES
HUMIC SUBSTANCES
RIBOSOMAL-RNA
SOIL
ADSORPTION
BACTERIAL
AMPLIFICATION

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10.1111/j.1574-6941.2012.01325.x

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title = "Sensitive life detection strategies for low-biomass environments: optimizing extraction of nucleic acids adsorbing to terrestrial and Mars analogue minerals",

abstract = "The adsorption of nucleic acids to mineral matrixes can result in low extraction yields and negatively influences molecular microbial ecology studies, in particular for low-biomass environments on Earth and Mars. We determined the recovery of nucleic acids from a range of minerals relevant to Earth and Mars. Clay minerals, but also other silicates and nonsilicates, showed very low recovery (",

keywords = "clays, DNA adsorption, DNA extraction, Mars analogue, mineralogy, subsurface, DNA EXTRACTION, QUANTITATIVE PCR, MICROBIAL-POPULATIONS, CONFORMATIONAL-CHANGES, HUMIC SUBSTANCES, RIBOSOMAL-RNA, SOIL, ADSORPTION, BACTERIAL, AMPLIFICATION",

author = "Direito, {Susana O. L.} and Andries Marees and Roling, {Wilfred F. M.}",

year = "2012",

month = jul,

doi = "10.1111/j.1574-6941.2012.01325.x",

language = "English",

volume = "81",

pages = "111--123",

journal = "FEMS Microbiology Ecology",

issn = "0168-6496",

publisher = "Wiley-Blackwell",

number = "1",

}

TY - JOUR

T1 - Sensitive life detection strategies for low-biomass environments

T2 - optimizing extraction of nucleic acids adsorbing to terrestrial and Mars analogue minerals

AU - Direito, Susana O. L.

AU - Marees, Andries

AU - Roling, Wilfred F. M.

PY - 2012/7

Y1 - 2012/7

N2 - The adsorption of nucleic acids to mineral matrixes can result in low extraction yields and negatively influences molecular microbial ecology studies, in particular for low-biomass environments on Earth and Mars. We determined the recovery of nucleic acids from a range of minerals relevant to Earth and Mars. Clay minerals, but also other silicates and nonsilicates, showed very low recovery (

AB - The adsorption of nucleic acids to mineral matrixes can result in low extraction yields and negatively influences molecular microbial ecology studies, in particular for low-biomass environments on Earth and Mars. We determined the recovery of nucleic acids from a range of minerals relevant to Earth and Mars. Clay minerals, but also other silicates and nonsilicates, showed very low recovery (

KW - clays

KW - DNA adsorption

KW - DNA extraction

KW - Mars analogue

KW - mineralogy

KW - subsurface

KW - DNA EXTRACTION

KW - QUANTITATIVE PCR

KW - MICROBIAL-POPULATIONS

KW - CONFORMATIONAL-CHANGES

KW - HUMIC SUBSTANCES

KW - RIBOSOMAL-RNA

KW - SOIL

KW - ADSORPTION

KW - BACTERIAL

KW - AMPLIFICATION

U2 - 10.1111/j.1574-6941.2012.01325.x

DO - 10.1111/j.1574-6941.2012.01325.x

M3 - Article

SN - 0168-6496

VL - 81

SP - 111

EP - 123

JO - FEMS Microbiology Ecology

JF - FEMS Microbiology Ecology

IS - 1

ER -

Sensitive life detection strategies for low-biomass environments: optimizing extraction of nucleic acids adsorbing to terrestrial and Mars analogue minerals

Abstract

Keywords / Materials (for Non-textual outputs)

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