Sensitive life detection strategies for low-biomass environments: optimizing extraction of nucleic acids adsorbing to terrestrial and Mars analogue minerals

Susana O. L. Direito, Andries Marees, Wilfred F. M. Roling*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

The adsorption of nucleic acids to mineral matrixes can result in low extraction yields and negatively influences molecular microbial ecology studies, in particular for low-biomass environments on Earth and Mars. We determined the recovery of nucleic acids from a range of minerals relevant to Earth and Mars. Clay minerals, but also other silicates and nonsilicates, showed very low recovery (

Original languageEnglish
Pages (from-to)111-123
Number of pages13
JournalFEMS Microbiology Ecology
Volume81
Issue number1
DOIs
Publication statusPublished - Jul 2012
Externally publishedYes

Keywords

  • clays
  • DNA adsorption
  • DNA extraction
  • Mars analogue
  • mineralogy
  • subsurface
  • DNA EXTRACTION
  • QUANTITATIVE PCR
  • MICROBIAL-POPULATIONS
  • CONFORMATIONAL-CHANGES
  • HUMIC SUBSTANCES
  • RIBOSOMAL-RNA
  • SOIL
  • ADSORPTION
  • BACTERIAL
  • AMPLIFICATION

Fingerprint

Dive into the research topics of 'Sensitive life detection strategies for low-biomass environments: optimizing extraction of nucleic acids adsorbing to terrestrial and Mars analogue minerals'. Together they form a unique fingerprint.

Cite this