Separating fluorescence from Raman spectra using a CMOS SPAD TCSPC line sensor for biomedical applications

Andrea Usai; Neil Finlayson; Christopher Gregory; Colin Campbell; Robert Henderson

doi:10.1117/12.2508459

Separating fluorescence from Raman spectra using a CMOS SPAD TCSPC line sensor for biomedical applications

Andrea Usai, Neil Finlayson, Christopher Gregory, Colin Campbell, Robert Henderson

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract / Description of output

A recently developed CMOS SPAD line sensor is used to separate Raman scattering from background fluorescence without the use of time-gating, relying entirely on the in-pixel time-correlated single photon counting (TCSPC). The system delivers both Raman and time-resolved fluorescence decay data and is being developed with the aim of evaluating the suitability of liver tissue for transplant surgery as their high fluorescence emission across a wide spectral range poses particular challenges to commercial Raman systems. The system is benchmarked using samples of pure distilled-water and common organic fluorophores, in comparison with results from a Renishaw InVia spectrometer.

Original language	English
Title of host publication	Paper 10873-26 Optical Biopsy XVII: Toward Real-Time Spectroscopic Imaging and Diagnosis
Publisher	SPIE
Volume	10873
DOIs	https://doi.org/10.1117/12.2508459
Publication status	Published - 6 Feb 2019

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@inproceedings{39c80587aa1f4131b5bd79e1051b33dc,

title = "Separating fluorescence from Raman spectra using a CMOS SPAD TCSPC line sensor for biomedical applications",

abstract = "A recently developed CMOS SPAD line sensor is used to separate Raman scattering from background fluorescence without the use of time-gating, relying entirely on the in-pixel time-correlated single photon counting (TCSPC). The system delivers both Raman and time-resolved fluorescence decay data and is being developed with the aim of evaluating the suitability of liver tissue for transplant surgery as their high fluorescence emission across a wide spectral range poses particular challenges to commercial Raman systems. The system is benchmarked using samples of pure distilled-water and common organic fluorophores, in comparison with results from a Renishaw InVia spectrometer.",

author = "Andrea Usai and Neil Finlayson and Christopher Gregory and Colin Campbell and Robert Henderson",

note = "Acceptance date set to exclude from REF OA Policy ",

year = "2019",

month = feb,

day = "6",

doi = "10.1117/12.2508459",

language = "English",

volume = "10873",

booktitle = "Paper 10873-26 Optical Biopsy XVII: Toward Real-Time Spectroscopic Imaging and Diagnosis",

publisher = "SPIE",

address = "United States",

}

Separating fluorescence from Raman spectra using a CMOS SPAD TCSPC line sensor for biomedical applications. / Usai, Andrea; Finlayson, Neil; Gregory, Christopher et al.
Paper 10873-26 Optical Biopsy XVII: Toward Real-Time Spectroscopic Imaging and Diagnosis. Vol. 10873 SPIE, 2019.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

TY - GEN

T1 - Separating fluorescence from Raman spectra using a CMOS SPAD TCSPC line sensor for biomedical applications

AU - Usai, Andrea

AU - Finlayson, Neil

AU - Gregory, Christopher

AU - Campbell, Colin

AU - Henderson, Robert

N1 - Acceptance date set to exclude from REF OA Policy

PY - 2019/2/6

Y1 - 2019/2/6

N2 - A recently developed CMOS SPAD line sensor is used to separate Raman scattering from background fluorescence without the use of time-gating, relying entirely on the in-pixel time-correlated single photon counting (TCSPC). The system delivers both Raman and time-resolved fluorescence decay data and is being developed with the aim of evaluating the suitability of liver tissue for transplant surgery as their high fluorescence emission across a wide spectral range poses particular challenges to commercial Raman systems. The system is benchmarked using samples of pure distilled-water and common organic fluorophores, in comparison with results from a Renishaw InVia spectrometer.

AB - A recently developed CMOS SPAD line sensor is used to separate Raman scattering from background fluorescence without the use of time-gating, relying entirely on the in-pixel time-correlated single photon counting (TCSPC). The system delivers both Raman and time-resolved fluorescence decay data and is being developed with the aim of evaluating the suitability of liver tissue for transplant surgery as their high fluorescence emission across a wide spectral range poses particular challenges to commercial Raman systems. The system is benchmarked using samples of pure distilled-water and common organic fluorophores, in comparison with results from a Renishaw InVia spectrometer.

U2 - 10.1117/12.2508459

DO - 10.1117/12.2508459

M3 - Conference contribution

VL - 10873

BT - Paper 10873-26 Optical Biopsy XVII: Toward Real-Time Spectroscopic Imaging and Diagnosis

PB - SPIE

ER -

Separating fluorescence from Raman spectra using a CMOS SPAD TCSPC line sensor for biomedical applications

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